Department of Biomedical Sciences, Atlantic Veterinary College University of Prince Edward Island, Prince Edward Island, Charlottetown, Canada.
Department of Companion Animals, Atlantic Veterinary College University of Prince Edward Island, Prince Edward Island, Charlottetown, Canada.
Sci Rep. 2018 Jul 6;8(1):10250. doi: 10.1038/s41598-018-28621-5.
Fish are a useful animal model for research, but our improvement in some aspects of their welfare has not kept pace with their increased popularity for this use. For example, researchers rarely use analgesics. We evaluated the side effects of lidocaine, a local anesthetic widely used in human and veterinary medicine. We infiltrated lidocaine on each side of the dorsal fin (total 20 mg/kg) of young rainbow trout (15 fish per group) compared with infiltration with an equal volume of saline. We monitored behaviour of individual trout during the 4-hour trial. Food was presented 5 times during the trial (30 min, 1 h, 2 h, 3 h, 4 h after infiltration) and we analyzed behaviour for 1 minute before and after food presentation. Behaviour of Saline-Infiltrated trout compared with trout that received no infiltration showed that infiltration in and of itself had no statistically significant effects on trout behaviour. However, there were many statistically significant effects of Lidocaine-Infiltrated trout compared with Saline-Infiltrated trout; none of the side-effects were adverse.
鱼类是一种有用的动物模型，可用于研究，但我们在提高鱼类福利的某些方面的进展，并未跟上人们对鱼类的这种用途的日益增加的需求。例如，研究人员很少使用止痛药。我们评估了利多卡因（一种在人类和兽医医学中广泛使用的局部麻醉剂）的副作用。我们将利多卡因渗透到幼年虹鳟鱼（每组 15 条鱼）的背鳍两侧（总剂量为 20mg/kg），与等量的盐水渗透进行了比较。我们在 4 小时的试验期间监测了个体虹鳟鱼的行为。在试验期间（渗透后 30 分钟、1 小时、2 小时、3 小时和 4 小时）共投喂 5 次食物，并在投喂前和投喂后 1 分钟分析行为。与未渗透的虹鳟鱼相比，盐水渗透的虹鳟鱼的行为表明，渗透本身对虹鳟鱼的行为没有统计学上的显著影响。然而，与盐水渗透的虹鳟鱼相比，利多卡因渗透的虹鳟鱼有许多统计学上显著的影响；没有任何副作用是不利的。
