一种来自弗氏柠檬酸杆菌的新型位点特异性内切脱氧核糖核酸酶。
A new site-specific endodeoxyribonuclease from Citrobacter freundii.
作者信息
Janulaitis A, Stakenas P
出版信息
FEBS Lett. 1983 Sep 19;161(2):210-2. doi: 10.1016/0014-5793(83)81009-6.
PMID:6617873
Abstract
Cfr10 I, a site-specific endonuclease from Citrobacter freundii strain RFL10, was isolated. It recognizes and cleaves the family of related sequences: 5'Pu decreases CCGGPy to generate DNA fragments with 5' tetranucleotide extensions. Cfr10 I may be useful in molecular cloning experiments, especially in conjunction with other enzymes which generate the same terminal extensions.
摘要
从弗氏柠檬酸杆菌菌株RFL10中分离出了位点特异性内切酶Cfr10 I。它识别并切割相关序列家族：5'嘌呤降低CCGG嘧啶以产生具有5'四核苷酸延伸的DNA片段。Cfr10 I可能在分子克隆实验中有用，特别是与产生相同末端延伸的其他酶一起使用时。
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