一种来自弗氏柠檬酸杆菌的新型限制性内切酶。
A new restriction endonuclease from Citrobacter freundii.
作者信息
Janulaitis A A, Stakenas P S, Lebedenko E N
出版信息
Nucleic Acids Res. 1982 Oct 25;10(20):6521-30. doi: 10.1093/nar/10.20.6521.
Abstract
CfrI, a new restriction endonuclease of unique substrate specificity, has been isolated from a Citrobacterfreundii strain. The enzyme recognizes a degenerated sequence PyGGCCPu in double-strand DNA and cleaves it between Py and G residues to yield 5' -protruding tetranucleotide ends GGCC.
摘要
CfrI是一种具有独特底物特异性的新型限制性内切酶，已从弗氏柠檬酸杆菌菌株中分离出来。该酶识别双链DNA中的简并序列PyGGCCPu，并在Py和G残基之间切割，产生5'-突出的四核苷酸末端GGCC。
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