Endo T, Hidaka H
FEBS Lett. 1983 Sep 19;161(2):235-8. doi: 10.1016/0014-5793(83)81015-1.
S-100 protein was examined for its ability to affect microtubule assembly-disassembly in vitro. Addition of S-100 protein inhibited microtubule assembly in a dose-dependent manner in the presence of Ca2+, but this effect was not observed in the presence of 2 mM EGTA. The interaction of microtubule proteins with S-100 protein was also demonstrated by Ca+-dependent affinity chromatography on S-100 protein-Sepharose. The proteins bound to the Sepharose column comigrated with tubulin as judged by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. These results might suggest a possible physiological role of S-100 protein in the microtubule assembly-disassembly system.
研究了S-100蛋白在体外影响微管组装-解聚的能力。在存在Ca2+的情况下，添加S-100蛋白以剂量依赖的方式抑制微管组装，但在存在2 mM乙二醇双（2-氨基乙基醚）四乙酸（EGTA）的情况下未观察到这种效应。通过在S-100蛋白-琼脂糖上进行Ca+依赖性亲和层析也证明了微管蛋白与S-100蛋白的相互作用。根据十二烷基硫酸钠-聚丙烯酰胺凝胶电泳判断，与琼脂糖柱结合的蛋白质与微管蛋白一起迁移。这些结果可能提示S-100蛋白在微管组装-解聚系统中可能具有生理作用。
