Smakman J G, Pijpker B A
J Neurosci Methods. 1983 Aug;8(4):365-73. doi: 10.1016/0165-0270(83)90093-6.
This paper describes a feedback system in which a neutral density wedge automatically controls the light flux applied to a photoreceptor, so that an intracellular recorded response is held constant. We describe here a "differential sampler" that subtracts the photoreceptors dark response from the light response induced by a chopped light source. The resulting signal drives the feedback system. The response time of the feedback system can be adjusted with a "digital integrator" to match a range of variable conditions that occur in different experiments.
本文描述了一种反馈系统,其中中性密度楔形物自动控制施加到光感受器的光通量,以便使细胞内记录的反应保持恒定。我们在此描述一种“差分采样器”,它从斩波光源诱导的光反应中减去光感受器的暗反应。所得信号驱动反馈系统。反馈系统的响应时间可以用“数字积分器”进行调整,以匹配不同实验中出现的一系列可变条件。