一种用于通过“光电压钳”研究光感受器适应性的数字反馈控制器应用。

A digital feedback controller application for studying photoreceptor adaptation by 'voltage clamp by light'.

作者信息

Djupsund K, Kouvalainen E, Järvilehto M, Weckström M

机构信息

Department of Physiology, University of Oulu, Finland.

出版信息

J Neurosci Methods. 1995 Nov;62(1-2):29-36. doi: 10.1016/0165-0270(95)00051-8.

DOI:10.1016/0165-0270(95)00051-8

PMID:8750082

Abstract

We present a new digital feedback application for the study of the sensitivity characteristics of photoreceptors. The amplitude of the recorded membrane voltage of a cell is steered by changing the incoming light intensity with a motor-driven circular, linear neutral-density wedge (CFW). The voltage response is sampled and fed to a software position controller of the CFW. The controller determines the position of the wedge according to the desired (command) value of the response. The light intensity changes during steady-state represent the sensitivity change, the time-course of adaptation.

摘要

我们提出了一种用于研究光感受器敏感性特征的新型数字反馈应用。通过使用电机驱动的圆形线性中性密度楔形物（CFW）改变入射光强度，来控制细胞记录膜电压的幅度。对电压响应进行采样，并将其馈送到CFW的软件位置控制器。控制器根据响应的期望（命令）值确定楔形物的位置。稳态期间的光强度变化代表敏感性变化，即适应的时间过程。