Forest C L
Exp Cell Res. 1983 Oct;148(1):143-54. doi: 10.1016/0014-4827(83)90194-5.
Mating interactions between opposite mating type gametes in Chlamydomonas reinhardi can be conditionally manipulated by the use of mutants which control different stages in this process. Two independently isolated, sex-limited mutants (expressed only in mating type (-) (mt-] gam-10 and gam-11, have been shown to display a temperature-sensitive defect in mating. Gametes of these mutants can agglutinate, signal, and participate in a specific contact between their activated mating structures and the mating structures of wild-type mt+ gametes but are unable to fuse at the restrictive temperature. Studies of these mutants have resulted in a clear demonstration of this mating type-specific interaction between activated mating structures in this organism. This contact is trypsin-sensitive and therefore appears to involve protein. Both this interaction, and the defect in mating structure fusion, can be further analysed using these mutants.
莱茵衣藻中不同交配型配子之间的交配相互作用可以通过使用控制该过程不同阶段的突变体来进行条件性操纵。两个独立分离的性别受限突变体（仅在交配型（-）（mt-）中表达）gam-10和gam-11，已被证明在交配中表现出温度敏感缺陷。这些突变体的配子可以凝集、发出信号，并在其活化的交配结构与野生型mt+配子的交配结构之间参与特定接触，但在限制温度下无法融合。对这些突变体的研究清楚地证明了该生物体中活化交配结构之间这种交配型特异性相互作用。这种接触对胰蛋白酶敏感，因此似乎涉及蛋白质。利用这些突变体可以进一步分析这种相互作用以及交配结构融合缺陷。
