Forest C L, Togasaki R K
Mol Gen Genet. 1977 Jun 8;153(2):227-30. doi: 10.1007/BF00264740.
A new selection procedure has beed developed for the isolation of temperature sensitive gametogenic mutants in Chlamydomonas reinhardi. This technique makes use of pet-10-1, a mutant strain incapable of photosynthetic electron transport, and the redox dye, methyl viologen. Two mutants found by this method are discussed. The first, gam-4, does not agglutinate at 35 degrees, whereas the second, gam-5, is capable of sexual agglutination, but not of zygote formation at the restrictive temperature. Both mutants appear to be inherited in a Mendelian fashion and are expressed in mating type (+) and mating type (-).
已经开发出一种新的筛选程序，用于分离莱茵衣藻中对温度敏感的配子发生突变体。该技术利用了pet-10-1，一种不能进行光合电子传递的突变菌株，以及氧化还原染料甲基紫精。讨论了通过这种方法发现的两个突变体。第一个是gam-4，在35摄氏度时不凝集，而第二个是gam-5，能够进行有性凝集，但在限制温度下不能形成合子。这两个突变体似乎都以孟德尔方式遗传，并且在交配型（+）和交配型（-）中都有表达。
