Goodenough U W, Adair W S, Caligor E, Forest C L, Hoffman J L, Mesland D A, Spath S
Soc Gen Physiol Ser. 1980;34:131-52.
Our investigations of the mating reaction of Chlamydomonas revealed a surprisingly intricate series of interrelated events. Adhering sites are moved to the flagellar tips in a fashion highly reminiscent of the capping of surface ligands over the centriolar regions of lymphocytes (28). Tipping is prevented by the gam-1 mutation and by agents that interact with tubulin; the molecular mechanism(s) for the inhibition effects are currently being sought. Tip locking appears to be accompanied by the accumulation of a dense material beneath the tip membrane, a postulated alteration of axonemal structure, and an immobilization of component(s) involved in surface motility. Two mating signals are then transduced to the locked-in cells who respond by shedding cell walls, activating mating structures, and fusing together. Signal transmission and/or reception is sensitive to such agents as trypsin, chymotrypsin, and cold temperature. Once zygotic cell fusion has occurred, tip unlocking and a reversal of the tip activation response appear to occur in parallel. Since all of these events can occur within 30 sec, the mating reaction serves as an experimental paradigm for studying rapid cellular responses to specific membrane-membrane interactions.
我们对衣藻交配反应的研究揭示了一系列惊人复杂且相互关联的事件。附着位点以一种与淋巴细胞中心粒区域表面配体加帽高度相似的方式移向鞭毛尖端（28）。gam - 1突变以及与微管蛋白相互作用的试剂可阻止尖端形成；目前正在探寻这种抑制作用的分子机制。尖端锁定似乎伴随着尖端膜下方致密物质的积累、轴丝结构的假定改变以及参与表面运动的成分的固定。然后，两个交配信号被传递给锁定的细胞，这些细胞通过脱落细胞壁、激活交配结构并融合在一起做出反应。信号传递和/或接收对诸如胰蛋白酶、胰凝乳蛋白酶和低温等试剂敏感。一旦发生合子细胞融合，尖端解锁和尖端激活反应的逆转似乎会同时发生。由于所有这些事件都可在30秒内发生，交配反应成为研究细胞对特定膜 - 膜相互作用的快速反应的实验范例。
