Sletten K, Kolberg J
Hoppe Seylers Z Physiol Chem. 1983 Aug;364(8):1047-51. doi: 10.1515/bchm2.1983.364.2.1047.
The complete primary structure of the alpha chain of the glucose- and mannose-specific lectin from Lathyrus sativus seeds has been determined. The structure was elucidated by Edman degradation on the whole chain as well as on fragments derived from cleaving the chain with BNPS-skatole and of peptides derived after tryptic digestion of the citraconylated chain. The analyses revealed a heterogeneity in the chain length (53 or 55 residues) and a microheterogeneity in positions 47 (aspartic acid and glycine). The sequence was highly homologous to the alpha chain of the lectin from Lathyrus odoratus.
已确定香豌豆种子中葡萄糖和甘露糖特异性凝集素α链的完整一级结构。通过对整条链以及用BNPS-粪臭素裂解该链得到的片段和对柠康酰化链进行胰蛋白酶消化后得到的肽段进行埃德曼降解来阐明该结构。分析揭示了链长度的异质性（53或55个残基）以及47位的微异质性（天冬氨酸和甘氨酸）。该序列与香豌豆凝集素的α链高度同源。
