禽成髓细胞瘤病毒RNA指导的DNA聚合酶对引物的识别
Primer recognition by avian myeloblastosis virus RNA-directed DNA polymerase.
作者信息
Brown R D, Armentrout R W
出版信息
J Virol. 1977 Mar;21(3):1236-9. doi: 10.1128/JVI.21.3.1236-1239.1977.
Abstract
Tryptophanyl-tRNA was specifically labeled at the 3' end with [3H]tryptophan and cleaved in half with RNase under denaturing conditions, and the 3' half was shown to hybridize exclusively at the 5' end of avian myeloblastosis virus RNA. The RNA-dependent DNA polymerase of avian myeloblastosis virus is capable of efficiently binding the 3' half of the primer molecule.
摘要
色氨酰 - tRNA在3'端用[³H]色氨酸进行特异性标记，并在变性条件下用核糖核酸酶切成两半，结果显示3'半段仅在禽成髓细胞瘤病毒RNA的5'端杂交。禽成髓细胞瘤病毒的RNA依赖性DNA聚合酶能够有效结合引物分子的3'半段。
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