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禽成髓细胞瘤逆转录酶使色氨酰 - tRNA脱酰基。

Avian myeloblastosis reverse transcriptase deacylates tryptophanyl-tRNA.

作者信息

Sarih L, Fournier M, von der Haar F, Labouesse J, Litvak S

出版信息

Nucleic Acids Res. 1982 Nov 25;10(22):7387-93. doi: 10.1093/nar/10.22.7387.

DOI:10.1093/nar/10.22.7387
PMID:6185923
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC327011/
Abstract

Tryptophanyl-tRNA can be used as a primer for RNA-dependent DNA synthesis by avian reverse transcriptase. We have determined that whereas the retroviral polymerase is not by itself capable of deacylating Trp-tRNATrp, the ester linkage between the 3' OH of the ribose moiety and the aminoacid can be very efficiently hydrolyzed when both the polymerase and the viral 35 S RNA are present.

摘要

色氨酰 - tRNA可被禽逆转录酶用作依赖RNA的DNA合成的引物。我们已经确定,虽然逆转录病毒聚合酶本身不能使色氨酰 - tRNA去酰化,但当聚合酶和病毒35S RNA都存在时,核糖部分3'羟基与氨基酸之间的酯键可以非常有效地被水解。

相似文献

1
Avian myeloblastosis reverse transcriptase deacylates tryptophanyl-tRNA.禽成髓细胞瘤逆转录酶使色氨酰 - tRNA脱酰基。
Nucleic Acids Res. 1982 Nov 25;10(22):7387-93. doi: 10.1093/nar/10.22.7387.
2
Specific binding of tryptophan transfer RNA to avian myeloblastosis virus RNA-dependent DNA polymerase (reverse transcriptase).色氨酸转移RNA与禽成髓细胞瘤病毒RNA依赖性DNA聚合酶(逆转录酶)的特异性结合。
Proc Natl Acad Sci U S A. 1975 Jul;72(7):2535-9. doi: 10.1073/pnas.72.7.2535.
3
tRNATrp (bovine) binding to the reverse transcriptase of avian myeloblastosis virus and function as a heterologous primer.牛源色氨酸转运RNA(tRNATrp)与禽成髓细胞瘤病毒逆转录酶结合并作为异源引物发挥作用。
Proc Natl Acad Sci U S A. 1977 May;74(5):1889-93. doi: 10.1073/pnas.74.5.1889.
4
Primer recognition by avian myeloblastosis virus RNA-directed DNA polymerase.禽成髓细胞瘤病毒RNA指导的DNA聚合酶对引物的识别
J Virol. 1977 Mar;21(3):1236-9. doi: 10.1128/JVI.21.3.1236-1239.1977.
5
Study of the interactions between avian myeloblastosis virus reverse transcriptase and primer tRNA. Affinity labeling and inactivation of the enzyme by periodate-treated tRNATrp.禽成髓细胞瘤病毒逆转录酶与引物tRNA相互作用的研究。经高碘酸盐处理的tRNATrp对该酶的亲和标记及失活作用。
Nucleic Acids Res. 1980 Sep 11;8(17):4009-20. doi: 10.1093/nar/8.17.4009.
6
Reverse transcriptase mediated binding of primer tRNA to the viral genome.逆转录酶介导引物tRNA与病毒基因组的结合。
Nucleic Acids Res. 1979 Aug 24;6(12):3831-43. doi: 10.1093/nar/6.12.3831.
7
Primer tRNATrp enhances the inhibition of avian myeloblastosis virus reverse transcriptase by pyridoxal-5'-phosphate.引物tRNATrp增强了磷酸吡哆醛对禽成髓细胞瘤病毒逆转录酶的抑制作用。
Biochem Biophys Res Commun. 1979 May 14;88(1):9-15. doi: 10.1016/0006-291x(79)91689-9.
8
Evidence for circularization of the avian oncornavirus RNA genome during proviral DNA synthesis from studies of reverse transcription in vitro.通过体外逆转录研究获得的证据表明,禽肿瘤病毒RNA基因组在原病毒DNA合成过程中发生环化。
Proc Natl Acad Sci U S A. 1976 Apr;73(4):1329-32. doi: 10.1073/pnas.73.4.1329.
9
Enzymatic synthesis of duplex DNA by avian myeloblastosis viral reverse transcriptase.禽成髓细胞瘤病毒逆转录酶催化合成双链DNA
Gene Amplif Anal. 1981;2:1-16.
10
Photochemical cross-linking studies on the interactions of avian myeloblastosis virus reverse transcriptase with primer tRNATrp and TTP.关于禽成髓细胞瘤病毒逆转录酶与引物tRNATrp和TTP相互作用的光化学交联研究。
Arch Biochem Biophys. 1980 Dec;205(2):437-48. doi: 10.1016/0003-9861(80)90127-7.

本文引用的文献

1
Photochemical cross-linking studies on the interactions of avian myeloblastosis virus reverse transcriptase with primer tRNATrp and TTP.关于禽成髓细胞瘤病毒逆转录酶与引物tRNATrp和TTP相互作用的光化学交联研究。
Arch Biochem Biophys. 1980 Dec;205(2):437-48. doi: 10.1016/0003-9861(80)90127-7.
2
Reverse transcriptase as the major determinant for selective packaging of tRNA's into Avian sarcoma virus particles.逆转录酶作为tRNA选择性包装进禽肉瘤病毒颗粒的主要决定因素。
J Virol. 1980 Dec;36(3):692-700. doi: 10.1128/JVI.36.3.692-700.1980.
3
Study of the interactions between avian myeloblastosis virus reverse transcriptase and primer tRNA. Affinity labeling and inactivation of the enzyme by periodate-treated tRNATrp.禽成髓细胞瘤病毒逆转录酶与引物tRNA相互作用的研究。经高碘酸盐处理的tRNATrp对该酶的亲和标记及失活作用。
Nucleic Acids Res. 1980 Sep 11;8(17):4009-20. doi: 10.1093/nar/8.17.4009.
4
Studies on the mechanism of deacylation of aminoacyl-tRNAs by aminoacyl-tRNA synthetases in the absence of adenosine monophosphate and pyrophosphate.在无磷酸腺苷和焦磷酸情况下,对氨酰-tRNA合成酶催化氨酰-tRNA脱酰基化机制的研究。
Biochimie. 1974;56(4):541-5. doi: 10.1016/s0300-9084(74)80071-4.
5
Structural studies on phenylalanine transfer ribonucleic acid from yeast with the spectroscopic label formycin.用光谱标记物间型霉素对酵母苯丙氨酸转移核糖核酸的结构研究
Eur J Biochem. 1974 Apr 16;43(3):617-25. doi: 10.1111/j.1432-1033.1974.tb03449.x.
6
Enzymatic incorporation of ATP and CTP analogues into the 3' end of tRNA.将ATP和CTP类似物酶促掺入tRNA的3'末端。
Eur J Biochem. 1977 Dec;81(3):579-89. doi: 10.1111/j.1432-1033.1977.tb11985.x.
7
Retroviruses.逆转录病毒
Annu Rev Biochem. 1978;47:35-88. doi: 10.1146/annurev.bi.47.070178.000343.
8
Control of protein synthesis in mammalian cells by aminoacylation of transfer ribonucleic acid.通过转移核糖核酸的氨酰化作用对哺乳动物细胞中蛋白质合成的控制。
Biochim Biophys Acta. 1979 Dec 17;565(2):293-304. doi: 10.1016/0005-2787(79)90206-5.
9
Influence of magnesium on the steady-state-derived order of substrate addition and product release in tRNATrp aminoacylation by beef pancreas tryptophan: tRNA ligase: significance of the deduced mechanism.
Eur J Biochem. 1978 Jul 3;87(3):541-50. doi: 10.1111/j.1432-1033.1978.tb12405.x.
10
Nucleotide sequence that binds primer for DNA synthesis to the avian sarcoma virus genome.将用于DNA合成的引物与禽肉瘤病毒基因组结合的核苷酸序列。
J Virol. 1976 Aug;19(2):548-58. doi: 10.1128/JVI.19.2.548-558.1976.