Trowbridge E A, Martin J F, Slater D N, Kishk Y T, Warren C W
Thromb Res. 1983 Jul 15;31(2):329-50. doi: 10.1016/0049-3848(83)90335-3.
The platelet volume distribution was measured in rat and rabbit in normal steady state platelet production. The animals were then sacrificed and the planimetric megakaryocyte and nuclear areas in each animal were measured using histological techniques. These areas were used to obtain an estimate of the megakaryocyte, megakaryocyte cytoplasm and nuclear volume distributions. The production, by physical fragmentation, of the platelet population from the measured megakaryocyte cytoplasm volume distribution was simulated on a computer. The platelet volume distribution predicted by physical fragmentation was then compared with the measured circulating platelet volume distribution from each animal. The physical fragmentation theory gave an accurate quantitative prediction of the observed platelet volume distribution over the whole volume range. Furthermore fragmentation theory predicted the mean platelet volume, the mode, the maximum frequency of the platelet volume distribution and the range of this distribution. Autopsy studies of megakaryocytes volume distributions in healthy man were fragmented to obtain predicted normal platelet volume distributions from the computer simulation. These distributions were compared with measured circulating platelet volume distributions from apparently healthy men who had a similar mean platelet volume. The platelet volume distribution predicted by physical fragmentation was again in quantitative agreement with the measured distribution over the whole volume range. This study provides further evidence that platelet production from megakaryocyte cytoplasm is by physical fragmentation. Furthermore the computer simulation suggests that the mode of production has a specific form. It also explains why different mammals have different mean platelet volumes.
在正常稳态血小板生成过程中，对大鼠和兔子的血小板体积分布进行了测量。然后处死这些动物，使用组织学技术测量每只动物的平面巨核细胞和核面积。这些面积用于估计巨核细胞、巨核细胞细胞质和核体积分布。通过物理破碎，根据测量的巨核细胞细胞质体积分布，在计算机上模拟血小板群体的生成。然后将物理破碎预测的血小板体积分布与每只动物测量的循环血小板体积分布进行比较。物理破碎理论在整个体积范围内对观察到的血小板体积分布给出了准确的定量预测。此外，破碎理论预测了平均血小板体积、众数、血小板体积分布的最大频率以及该分布的范围。对健康人巨核细胞体积分布的尸检研究进行了破碎处理，以通过计算机模拟获得预测的正常血小板体积分布。将这些分布与平均血小板体积相似的明显健康男性测量的循环血小板体积分布进行比较。物理破碎预测的血小板体积分布在整个体积范围内再次与测量分布在数量上一致。这项研究进一步证明，巨核细胞细胞质产生血小板是通过物理破碎。此外，计算机模拟表明生成模式具有特定形式。它还解释了为什么不同的哺乳动物有不同的平均血小板体积。
