Trowbridge E A, Martin J F, Slater D N, Kishk Y T, Warren C W, Harley P J, Woodcock B
Clin Phys Physiol Meas. 1984 Aug;5(3):145-70. doi: 10.1088/0143-0815/5/3/007.
Platelet count and volume were measured in man (n = 51) and rat (n = 9). Bone marrow megakaryocyte nuclear and megakaryocyte planimetric areas were measured in man (n = 11) and rat (n = 9). Megakaryocyte cytoplasmic volumes were computed from the planimetric areas. Rat had a significantly higher (p less than 0.001) mean megakaryocyte cytoplasmic volume than man and a significantly wider range (p less than 0.01). Rat mean platelet volume was significantly lower (p less than 0.001) than man while the rat platelet count was significantly higher (p less than 0.001) than man. A computer simulation of the random binary sequential division of megakaryocyte cytoplasm was used to explain these observations. Transmission electron microscopy shows that the site of this binary sequential division is probably the pulmonary circulation. The number of circulating megakaryocytes ml-1 of blood which would maintain the observed platelet counts was computed. The ellipses of constant density associated with the bivariate Gaussian distribution of platelet count and mean volume were computed. Platelet volume distributions of 13 men within two standard deviations of the composite mean of platelet count and mean volume were used to construct the platelet volume distribution signature in normal man. A similar platelet volume distribution signature for rat was constructed. The two distributions were significantly different. Neither rat nor man had a log Gaussian platelet volume distribution, however the measured volume distributions tended towards a log Gaussian curve.
对51名人类受试者和9只大鼠进行了血小板计数和体积测量。对11名人类受试者和9只大鼠测量了骨髓巨核细胞核面积和巨核细胞平面测量面积。巨核细胞胞质体积由平面测量面积计算得出。大鼠的平均巨核细胞胞质体积显著高于人类（p<0.001），且范围显著更宽（p<0.01）。大鼠的平均血小板体积显著低于人类（p<0.001），而大鼠的血小板计数显著高于人类（p<0.001）。利用计算机模拟巨核细胞胞质的随机二元顺序分裂来解释这些观察结果。透射电子显微镜显示，这种二元顺序分裂的部位可能是肺循环。计算了维持观察到的血小板计数所需的每毫升血液中循环巨核细胞的数量。计算了与血小板计数和平均体积的双变量高斯分布相关的等密度椭圆。使用13名男性血小板计数和平均体积综合平均值的两个标准差范围内的血小板体积分布来构建正常男性的血小板体积分布特征。构建了类似的大鼠血小板体积分布特征。这两种分布有显著差异。大鼠和人类均无对数高斯血小板体积分布，然而测量的体积分布趋向于对数高斯曲线。
