Klitsunova N V, Gosteva V V, Hernández-Verden D
Vopr Virusol. 1983 Jul-Aug;28(4):84-7.
Primary monkey kidney cells were enucleated by the combined effect of cytochalasin B and ultracentrifugation. The effectiveness of enucleation in the experiments was 96-99%. Cytoplasts after 1-hour restoration were inoculated with the WR strain of vaccinia virus at a multiplicity of 0.1, 1.0, 10, and 50 PFU/cell. Within 20-21 h postinoculation when a marked cytopathic effect of the virus was evident, the material was fixed for electron microscopy. Serial ultrathin sections of the infected material were examined. Careful examination of the infected cytoplasts revealed all the usual stages of the morphogenetic cycle of vaccinia virus, from virion absorption by the host cell to clumps of mature virus particles in the cytoplasm and extracellularly. Vaccinia virus is thought to have morphologically complete replication cycle in the cytoplasts.
通过细胞松弛素B和超速离心的联合作用去除原代猴肾细胞的细胞核。实验中去核的成功率为96% - 99%。将恢复1小时后的胞质体分别以每细胞0.1、1.0、10和50个蚀斑形成单位（PFU）的接种量接种痘苗病毒WR株。接种后20 - 21小时，当病毒出现明显的细胞病变效应时，取材料进行电子显微镜固定。对感染材料的系列超薄切片进行检查。仔细检查感染的胞质体发现了痘苗病毒形态发生周期的所有常见阶段，从宿主细胞吸收病毒粒子到细胞质和细胞外成熟病毒粒子团块的形成。痘苗病毒被认为在胞质体中具有形态完整的复制周期。
