Shainkin-Kestenbaum R, Winikoff Y, Chaimovitz C, Zimlichman S, Sarov I
Department of Nephrology, Soroka Medical Center, Beer Sheva, Israel.
Isr J Med Sci. 1993 Jan;29(1):2-6.
Kidney cell lines MA104 and BGM were infected with vaccinia and measles viruses respectively in the presence of 45Ca. Increased 45Ca level was detected in the virally infected cells as compared with control cells. An enhancement of 28 +/- 6% and 37 +/- 13% was shown in vaccinia and measles respectively following 5 h of infection. The effect of the calcium antagonist verapamil was studied in both vaccinia- and measles-infected cells. In one-step growth experiments, the mean inhibitory effect of 90 microM verapamil on viral yield after 13 h was 97 +/- 1% in the case of vaccinia. In the measles virus after 47 h a mean of 76 +/- 5% inhibition was detected. The suitability of verapamil as a potential antiviral agent is suggested and requires further investigation.
在存在45Ca的情况下，肾细胞系MA104和BGM分别感染了痘苗病毒和麻疹病毒。与对照细胞相比，在病毒感染的细胞中检测到45Ca水平升高。感染5小时后，痘苗病毒和麻疹病毒分别显示出28±6%和37±13%的增强。在痘苗病毒和麻疹病毒感染的细胞中都研究了钙拮抗剂维拉帕米的作用。在一步生长实验中，90微摩尔维拉帕米在13小时后对痘苗病毒产量的平均抑制作用为97±1%。在麻疹病毒感染47小时后，检测到平均76±5%的抑制作用。提示维拉帕米作为一种潜在抗病毒剂的适用性，需要进一步研究。
