Shi Q X, Friend D S
Biol Reprod. 1983 Nov;29(4):1027-32. doi: 10.1095/biolreprod29.4.1027.
The effect of gossypol acetate at various concentrations (10(-6) to 10(-4) M) on guinea pig sperm forward progressive movement, capacitation, and the acrosome reaction was explored in vitro. We found that 10(-4) M gossypol completely abolished the forward progressive motility of the sperm, and that this inhibition of motility was proportional to the concentration of gossypol used. Also, a dose-dependent decrease in acrosome reactions occurred with concentrations of the agent as low as 5.0 X 10(-6) M. However, we observed that such prevention of the acrosome reaction apparently happens at the capacitation stage rather than during the acrosome reaction itself. Inhibition of capacitation by gossypol was reversible--once the spermatozoa were capacitated in gossypol-free medium, the compound did not block the reaction.
体外研究了不同浓度（10⁻⁶至10⁻⁴M）的醋酸棉酚对豚鼠精子向前运动、获能及顶体反应的影响。我们发现，10⁻⁴M的醋酸棉酚完全消除了精子的向前运动能力，且这种运动抑制与所用棉酚的浓度成正比。此外，当该试剂浓度低至5.0×10⁻⁶M时，顶体反应呈剂量依赖性降低。然而，我们观察到这种对顶体反应的抑制显然发生在获能阶段而非顶体反应本身过程中。棉酚对获能的抑制是可逆的——一旦精子在无棉酚的培养基中获能，该化合物就不会阻断反应。
