West P
Clin Chem. 1983 Dec;29(12):2086-8.
I compared results for aspartate aminotransferase (EC 2.6.1.1) obtained with a reaction-rate analyzer (LKB 2086 Mark Two), based on IFCC methodology, and a continuous-flow analyzer (the Technicon SMA 2) for 115 patients' sera and seven commercial quality-control sera. The data from the SMA 2 showed a clear positive bias in those sera with activities exceeding 40 U/L (the upper limit of the reference range). Independent data to support the bias of the SMA 2 and other continuous-flow analyzer systems are presented. Application of a correction factor to the SMA 2 data above the upper limit of the range significantly decreased this bias. Failure to apply such a factor to data obtained from continuous-flow analyzers could lead to serious clinical misinterpretation.
我比较了用基于国际临床化学和检验医学联合会（IFCC）方法的反应速率分析仪（LKB 2086 Mark Two型）和连续流动分析仪（Technicon SMA 2型）检测115例患者血清及7份商业质量控制血清中天冬氨酸氨基转移酶（EC 2.6.1.1）的结果。SMA 2型分析仪的数据显示，在活性超过40 U/L（参考范围上限）的血清中存在明显的正偏差。文中给出了支持SMA 2型及其他连续流动分析仪系统偏差的独立数据。对超出范围上限的SMA 2型分析仪数据应用校正因子可显著降低这种偏差。对从连续流动分析仪获得的数据未应用该因子可能导致严重的临床误判。
