Chan J K, Shyamala G
Endocrinology. 1983 Dec;113(6):2202-9. doi: 10.1210/endo-113-6-2202.
The present studies were initiated to study peroxidase and its possible regulation by estrogen in normal mammary glands. The activity of peroxidase was measured biochemically using guaiacol as the substrate for oxidation. Significant levels of peroxidase activity were associated with the particulate fraction of mammary glands from virgin mice, pregnant mice, and mice undergoing lactational involution. However, during lactation there was no detectable level of peroxidase activity in the mammary glands. Although ovariectomy led to a decrease in mammary peroxidase, detailed studies using various hormonal manipulations revealed that mammary peroxidase was perhaps not a product of estrogen action alone, but might be the result of a complex hormonal control related to growth. Alternatively, a critical evaluation of all of the data obtained with mammary glands and a comparison of these data obtained with the uterus also suggest that the presence of peroxidase in mammary glands may be due to infiltration of eosinophils and macrophages in these tissues resulting from mast cell degranulation.
开展本研究是为了研究过氧化物酶及其在正常乳腺中可能受到的雌激素调节。以愈创木酚作为氧化反应的底物，通过生化方法测定过氧化物酶的活性。来自处女小鼠、怀孕小鼠和处于泌乳期退化阶段小鼠的乳腺颗粒部分与显著水平的过氧化物酶活性相关。然而，在泌乳期间，乳腺中未检测到过氧化物酶活性。虽然卵巢切除术导致乳腺过氧化物酶减少，但使用各种激素处理的详细研究表明，乳腺过氧化物酶可能并非仅由雌激素作用产生，而是可能与生长相关的复杂激素控制的结果。另外，对所有乳腺相关数据进行批判性评估，并将这些数据与子宫相关数据进行比较，也表明乳腺中过氧化物酶的存在可能是由于肥大细胞脱颗粒导致嗜酸性粒细胞和巨噬细胞浸润这些组织所致。
