Larkin E C, Goheen S C, Rao G A
Environ Res. 1983 Dec;32(2):445-54. doi: 10.1016/0013-9351(83)90124-x.
Monkeys (Macaca radiata) were exposed to a low dose (0.64 ppm) of ozone (O3) for 8 hr each day over a 1-year period. Control monkeys were exposed to filtered air. The morphology of the red blood cell (RBC) from these monkeys was analyzed by scanning electron microscopy. Red cells from control monkeys had normal morphology with 69% having biconcave shape (discocytes). In O3-exposed animals, blood contained only 35% discocytes. Knizocytes, stomatocytes, and spherocytes each have defined shape which are different from discocytes. The number of knizocytes and stomatocytes in O3-exposed monkeys was twice that of controls. In addition, significant levels of spherocytes were observed while they were absent in the blood of controls. The fatty acid composition of RBC from control and O3-exposed monkeys was the same. These were similar to that of human RBC. However, earlier reports of monkey RBC fatty acid composition differ from our results. These differences are discussed. We conclude that low-dose O3 exposure changes the morphology, but not the fatty acid composition, of erythrocytes in vivo.
在为期1年的时间里，每天让恒河猴（食蟹猴）暴露于低剂量（0.64 ppm）的臭氧（O₃）中8小时。对照猴暴露于过滤空气中。通过扫描电子显微镜分析这些猴子红细胞（RBC）的形态。对照猴的红细胞形态正常，69%呈双凹形（圆盘状红细胞）。在暴露于臭氧的动物中，血液中只有35%是圆盘状红细胞。棘状红细胞、口形红细胞和球形红细胞都有与圆盘状红细胞不同的特定形状。暴露于臭氧的猴子中棘状红细胞和口形红细胞的数量是对照组的两倍。此外，观察到有大量球形红细胞，而对照组血液中没有。对照猴和暴露于臭氧的猴子的红细胞脂肪酸组成相同。这些与人类红细胞的脂肪酸组成相似。然而，早期关于猴红细胞脂肪酸组成的报道与我们的结果不同。本文讨论了这些差异。我们得出结论，低剂量臭氧暴露会改变体内红细胞的形态，但不会改变其脂肪酸组成。
