Bowtell D D, Mitchell G F, Anders R F, Lightowlers M W, Rickard M D
Exp Parasitol. 1983 Dec;56(3):416-27. doi: 10.1016/0014-4894(83)90087-5.
Biosynthetically or exogenously labeled proteins and immunoprecipitated protein antigens of established 28-day-old larvae of Taenia taeniaeformis were compared with proteins and antigens of infective oncospheres using single and two-dimensional gel electrophoresis. Immunoprecipitation was carried out using sera from infected mice and mouse antisera raised to larvae or oncospheres, and emphasis was placed on identifying antigens common to both oncospheres and larvae. Two major larval antigens of Mr 40,000 and 200,000, designated Tt40 and Tt200, are common to somatic larval preparations and oncospheres. Additionally, two major oncosphere antigens of Mr 55,000 and 60,000, designated Tt55 and Tt60, are also present in larval excretory and secretory (i.e., ES or exoantigen) products. Information obtained from these immunoprecipitation analyses will facilitate isolation and production of common as well as stage-specific protein antigens in the development of defined-antigen vaccines in this model system of cysticercosis.
使用单向和双向凝胶电泳，将绦虫带绦虫28日龄成熟幼虫生物合成或外源标记的蛋白质以及免疫沉淀的蛋白质抗原与感染性六钩蚴的蛋白质和抗原进行比较。使用来自感染小鼠的血清以及针对幼虫或六钩蚴产生的小鼠抗血清进行免疫沉淀，重点是鉴定六钩蚴和幼虫共有的抗原。两种主要的幼虫抗原，分子量分别为40,000和200,000，命名为Tt40和Tt200，在幼虫体细胞制剂和六钩蚴中都存在。此外，两种主要的六钩蚴抗原，分子量分别为55,000和60,000，命名为Tt55和Tt60，也存在于幼虫排泄和分泌（即ES或外抗原）产物中。从这些免疫沉淀分析中获得的信息将有助于在该囊尾蚴病模型系统中开发特定抗原疫苗时分离和生产共同以及阶段特异性的蛋白质抗原。
