Bøgh H O, Rickard M D, Lightowlers M W
University of Melbourne, Veterinary Clinical Centre, Werribee, Victoria, Australia.
Parasite Immunol. 1988 May;10(3):255-64. doi: 10.1111/j.1365-3024.1988.tb00219.x.
The possible existence of stage-specific immune responses to Taenia taeniaeformis infection was investigated in C3H/He mice vaccinated with antigens prepared from either the oncosphere or metacestode stages. Mice were immunized twice, 2 weeks apart, with antigen in Freund's complete adjuvant. Two weeks after the second immunization they were challenged with 250 T. taeniaeformis eggs and killed day 0, 5, 10, 15, 20, 25, 30, 45 and 60 after infection. Gross examination of the livers revealed marked differences between oncosphere (TtO) and metacestode (TtM) vaccinated mice. Very few metacestodes were found in the first group but most of those that evaded the initial host attack developed like the cysts found in the control group. In contrast, many degenerating metacestodes were found in the TtM vaccinated group. In a subsequent experiment groups of mice were vaccinated with varying doses of either TtO or TtM to determine whether the qualitative differences observed above were due to antigen dose effects. However, varying antigen doses gave the same results. These data show that vaccination with oncospheres generates an immune response capable of killing invading larvae soon after infection whereas vaccination with TtM results in larvae being killed at a later stage, suggesting that there are stage-specific, host-protective antigens.
在用从绦虫蚴或中绦期制备的抗原接种的C3H/He小鼠中，研究了对鼠带绦虫感染的阶段特异性免疫反应的可能存在情况。小鼠用弗氏完全佐剂中的抗原免疫两次，间隔2周。第二次免疫后2周，它们用250个鼠带绦虫卵攻击，并在感染后第0、5、10、15、20、25、30、45和60天处死。对肝脏的大体检查显示，接种绦虫蚴（TtO）和接种中绦期（TtM）的小鼠之间存在明显差异。在第一组中发现的中绦期很少，但大多数逃避了初始宿主攻击的中绦期发育情况与对照组中发现的囊肿相似。相比之下，在接种TtM的组中发现了许多退化的中绦期。在随后的实验中，用不同剂量的TtO或TtM对小鼠组进行接种，以确定上述观察到的定性差异是否是由于抗原剂量效应。然而，不同的抗原剂量给出了相同的结果。这些数据表明，用绦虫蚴接种可产生一种免疫反应，能够在感染后不久杀死入侵的幼虫，而用TtM接种则导致幼虫在后期被杀死，这表明存在阶段特异性的宿主保护性抗原。
