Craig P S, Rickard M D
Z Parasitenkd. 1982;67(2):197-209. doi: 10.1007/BF00928115.
The antigenicity and specificity of crude antigens collected during the in vitro maintenance of Taenia hydatigena and T. ovis, excretory/secretory (ES) antigens, were assessed in a peroxidase microenzyme-linked immunosorbent assay (ELISA), using sera from lambs given experimental monospecific infections with T. hydatigena, T. ovis, Echinococcus granulosus or Fasciola hepatica. ES antigens of larval cysts of T. ovis and T. hydatigena were less reactive than those of adult or oncosphere stages. Strong interspecific cros-reactions occurred between all antigen preparations, and these antigens offered no better specificity than crude somatic extracts. IgG1 was the major immunoglobulin detected in sera from lambs experimentally infected with T. ovis or T. hydatigena using antigens prepared from sonicated oncospheres. Discrete peaks of anti-oncospheral antibodies were detected following initial and challenge infections with eggs (whether the homologous or heterologous species), when sera were assayed with a PBS sonicate or an ES antigen from oncospheres. However, when oncospheres solubilised with sodium deoxycholate were used, the antibody response was prolonged and resembled that reported previously when somatic extracts of adult and metacestode stages were used as antigen. The results showed that oncospheres share antigens in common with other life-cycle stages, but also support the notion that they may possess some unique stage-specific antigenic determinants.
在体外培养泡状带绦虫和绵羊绦虫期间收集的粗抗原、排泄/分泌（ES）抗原的抗原性和特异性，采用过氧化物酶微酶联免疫吸附测定（ELISA）进行评估，所用血清来自经实验性单特异性感染泡状带绦虫、绵羊绦虫、细粒棘球绦虫或肝片吸虫的羔羊。绵羊绦虫和泡状带绦虫幼虫囊肿的ES抗原的反应性低于成虫或六钩蚴阶段的抗原。所有抗原制剂之间均发生强烈的种间交叉反应，且这些抗原的特异性并不比粗体壁提取物更好。使用从超声处理的六钩蚴制备的抗原时，IgG1是在经实验感染绵羊绦虫或泡状带绦虫的羔羊血清中检测到的主要免疫球蛋白。在用卵进行初次感染和攻击感染后（无论同源或异源物种），当用PBS超声裂解物或来自六钩蚴的ES抗原检测血清时，可检测到抗六钩蚴抗体的离散峰。然而，当使用用脱氧胆酸钠溶解的六钩蚴时，抗体反应延长，类似于先前报道的使用成虫和中绦期的体壁提取物作为抗原时的情况。结果表明，六钩蚴与其他生命周期阶段共享抗原，但也支持它们可能具有一些独特的阶段特异性抗原决定簇的观点。
