Paulus S F, Grossie J
Diabetes. 1983 Nov;32(11):1035-9. doi: 10.2337/diab.32.11.1035.
Diabetes was produced by withholding insulin treatment from previously alloxanized female rats. Isometric contraction was assessed in soleus and extensor digitorum longus (EDL) muscles removed 2 h to 32 days after insulin withdrawal. Directly induced contractions were measured in vitro at 20 degrees C. In soleus muscles from severely diabetic rats, average twitch and tetanic forces were normal or slightly greater than that of controls of similar age, whereas in EDL, marked decreases appeared in both twitch and tetanic forces. Soleus muscle from severely diabetic rats was not depolarized as already reported in EDL. After 16 and 32 days in the diabetic state, soleus muscles from moderately diabetic rats generated average tetanic forces that were equal to that found in age-matched controls, whereas EDL tetanic forces were significantly (P = less than 0.01) weaker. Average specific twitch force in diabetic soleus muscles was greater than age-matched controls after 16 and 32 days in the diabetic state. In diabetic soleus muscle, significant increases in the average half relaxation time and twitch duration were seen after prolonged (16 and 32 days) periods of diabetes. No changes were seen in the same temporal parameters of the twitch in diabetic EDL muscle. A greater atrophy appeared in EDL than in soleus after 16 and 32 days of uncontrolled diabetes.
通过对先前用四氧嘧啶处理过的雌性大鼠停止胰岛素治疗来诱发糖尿病。在停止胰岛素治疗后2小时至32天取出的比目鱼肌和趾长伸肌（EDL）中评估等长收缩。在20摄氏度下于体外测量直接诱发的收缩。在严重糖尿病大鼠的比目鱼肌中，平均单收缩力和强直收缩力正常或略高于同龄对照组，而在EDL中，单收缩力和强直收缩力均显著下降。如先前在EDL中所报道的，严重糖尿病大鼠的比目鱼肌未发生去极化。在处于糖尿病状态16天和32天后，中度糖尿病大鼠的比目鱼肌产生的平均强直收缩力与年龄匹配的对照组相当，而EDL的强直收缩力则显著较弱（P < 0.01）。在处于糖尿病状态16天和32天后，糖尿病比目鱼肌的平均比单收缩力大于年龄匹配的对照组。在糖尿病比目鱼肌中，经过较长时间（16天和32天）的糖尿病后，平均半松弛时间和单收缩持续时间显著增加。糖尿病EDL肌单收缩的相同时间参数未见变化。在未控制的糖尿病状态16天和32天后，EDL比目鱼肌出现更严重的萎缩。
