Söderlund G, Kihlström E
Infect Immun. 1983 Dec;42(3):930-5. doi: 10.1128/iai.42.3.930-935.1983.
The kinetics of attachment and ingestion of Chlamydia trachomatis serotype L1 by monolayers of McCoy cells were studied by using a method that discriminated between attachment and uptake. When about 1% of the McCoy cells was infected, the proteinase K-resistant chlamydial fraction, regarded as ingested chlamydiae, reached a constant value after about 3 h of incubation at 37 degrees C. Uptake of chlamydiae at 4 degrees C could not be demonstrated. The attached and ingested chlamydial fractions were constant over an eightfold increase in chlamydial inoculum. Chitobiose and chitotriose, the di- and trisaccharides of N-acetyl-D-glucosamine, reduced the association of C. trachomatis serotype L1 with McCoy cells. Higher concentrations of chitobiose also selectively inhibited ingestion of chlamydiae. A corresponding effect of chitobiose was also observed on the number of chlamydial inclusions. Wheat germ agglutinin, specific for N-acetyl-D-glucosamine residues, reduced the association of chlamydiae when incubated at 4 degrees C, but not at 37 degrees C. A small inhibiting effect of concanavalin A on association of chlamydiae, but no effect of the corresponding carbohydrates, indicates a nonspecific effect on chlamydial attachment of this lectin. These results suggest that beta 1 leads to 4-linked oligomers of N-acetyl-D-glucosamine are important in the specificity of attachment of C. trachomatis to McCoy cells.
采用一种能够区分附着与摄取的方法，研究了沙眼衣原体L1血清型在 McCoy 细胞单层上的附着和摄取动力学。当约1%的 McCoy 细胞被感染时，在37℃孵育约3小时后，被认为是摄取的衣原体的蛋白酶K抗性衣原体部分达到恒定值。在4℃下无法证明衣原体的摄取。在衣原体接种量增加八倍的情况下，附着和摄取的衣原体部分保持恒定。N-乙酰-D-葡萄糖胺的二糖和三糖壳二糖和壳三糖，减少了沙眼衣原体L1血清型与 McCoy 细胞的结合。较高浓度的壳二糖也选择性地抑制衣原体的摄取。在衣原体包涵体数量上也观察到了壳二糖的相应作用。对 N-乙酰-D-葡萄糖胺残基具有特异性的麦胚凝集素，在4℃孵育时减少了衣原体的结合，但在37℃时没有。伴刀豆球蛋白A对衣原体结合有较小的抑制作用，但相应的碳水化合物没有作用，表明该凝集素对衣原体附着有非特异性作用。这些结果表明，N-乙酰-D-葡萄糖胺的β1-4连接寡聚体在沙眼衣原体与 McCoy 细胞附着的特异性中起重要作用。
