Sugarman B, Agbor P
Infection. 1987 Jan-Feb;15(1):35-9. doi: 10.1007/BF01646116.
Zinc was found to have profoundly different effects upon the infection of McCoy cells (mouse fibroblasts) by two strains of Chlamydia trachomatis dependent upon the time and concentration of zinc exposure. Radiolabeled zinc-65 became McCoy cell-associated in a manner independent of incubation temperature, but highly dependent on incubation time and zinc concentration. This effect was maximal after 30 to 60 minutes of incubation. Correspondingly, incubation of a chlamydia inoculant with McCoy cells and supplemental zinc (10(-5) to 10(-4) M) for 1 h was associated with significantly (approximately twofold) more binding of the chlamydia to the McCoy cells compared with control media (8 X 10(-6) M Zn). More prolonged incubation of the chlamydia and McCoy cells with supplemental zinc was associated with significantly fewer chlamydia inclusions. Concentrations of 5 X 10(-4) M zinc or higher were also found to be toxic to the McCoy cells after 48 h of incubation. Brief exposure to supplemental zinc may augment infection of cells by chlamydia: however, more prolonged exposure to the same concentrations of zinc lessens cellular infection by chlamydia.
研究发现，锌对两株沙眼衣原体感染 McCoy 细胞（小鼠成纤维细胞）的影响因锌暴露的时间和浓度而有显著差异。放射性标记的锌 -65 与 McCoy 细胞的结合方式与孵育温度无关，但高度依赖于孵育时间和锌浓度。这种影响在孵育 30 至 60 分钟后达到最大。相应地，将衣原体接种物与 McCoy 细胞及补充锌（10^(-5)至 10^(-4) M）一起孵育 1 小时，与对照培养基（8×10^(-6) M 锌）相比，衣原体与 McCoy 细胞的结合显著增加（约两倍）。衣原体和 McCoy 细胞与补充锌的孵育时间延长，衣原体包涵体显著减少。孵育 48 小时后，还发现 5×10^(-4) M 或更高浓度的锌对 McCoy 细胞有毒性。短暂暴露于补充锌可能会增强衣原体对细胞的感染：然而，长时间暴露于相同浓度的锌会减少衣原体对细胞的感染。
