Bose S K, Paul R G
J Gen Microbiol. 1982 Jun;128(6):1371-9. doi: 10.1099/00221287-128-6-1371.
A procedure has been developed to yield infectious elementary bodies of the lymphogranuloma venereum strains LGV 434 and 404 of Chlamydia trachomatis, labelled during intracellular growth in HeLa 229 cells. The final preparation, obtained after velocity sedimentation of a polycarbonate membrane-filtered sample through a sucrose gradient, is free of host proteins and, more importantly, of chlamydial reticulate bodies. Using such purified preparations, it was found that the association of LGV 434 elementary bodies with HeLa 229 cultures was unaffected by the pretreatment of the host cells with a variety of lectins or with neuraminidases from Clostridium perfringens and Vibrio cholerae. The association was inhibited by dextran sulphate and by mild trypsin treatment of HeLa cultures. Treatment of purified elementary bodies with trypsin, chymotrypsin, neuraminidases and a variety of carbohydrates and lectins did not produce any change in the rate of association with HeLa cultures. Heat-inactivated elementary bodies were significantly less able to associate with the host cells.
已开发出一种程序，可产生沙眼衣原体淋巴肉芽肿性病原体菌株LGV 434和404的感染性原体，这些原体在HeLa 229细胞内生长期间被标记。通过蔗糖梯度对聚碳酸酯膜过滤样品进行速度沉降后获得的最终制剂不含宿主蛋白，更重要的是不含衣原体网状体。使用这种纯化制剂发现，LGV 434原体与HeLa 229培养物的结合不受用多种凝集素或产气荚膜梭菌和霍乱弧菌神经氨酸酶对宿主细胞进行预处理的影响。硫酸葡聚糖和对HeLa培养物进行温和胰蛋白酶处理可抑制这种结合。用胰蛋白酶、胰凝乳蛋白酶、神经氨酸酶以及多种碳水化合物和凝集素处理纯化的原体，与HeLa培养物的结合速率没有任何变化。热灭活的原体与宿主细胞结合的能力明显降低。
