Wal J M, Peleran J C, Bortes G F
J Assoc Off Anal Chem. 1980 Sep;63(5):1044-8.
A rapid and sensitive high performance liquid chromatographic method for the determination of trace amounts of chloramphenicol (CP) in milk has been developed. The antibiotic can be quantitated at a 10 ppb level with a limit of detectability estimated at 5 ppb. Recoveries ranged from 72 to 99.5%. Milking studies have been carried out on goats that received CP either by intramuscular injection or by intramammary administration. CP was measured in milk samples collected 1, 2, 3, 4, 5, 6, 7, 8, 24, 32 h after treatments. No residue could be detected 32 h after treatment. No interfering peaks appeared on control milk chromatograms.
已开发出一种快速灵敏的高效液相色谱法，用于测定牛奶中痕量的氯霉素（CP）。该抗生素可在10 ppb的水平进行定量，估计检测限为5 ppb。回收率在72%至99.5%之间。已对通过肌肉注射或乳房内给药接受CP的山羊进行了挤奶研究。在处理后1、2、3、4、5、6、7、8、24、32小时采集的牛奶样品中测量了CP。处理后32小时未检测到残留。对照牛奶色谱图上未出现干扰峰。
