Selsted M E, Brown D M, DeLange R J, Lehrer R I
J Biol Chem. 1983 Dec 10;258(23):14485-9.
The microbicidal peptides, MCP-1 and MCP-2, of rabbit alveolar macrophages were purified by an improved procedure that employed preparative gel electrophoresis and high performance liquid chromatography. The peptides were arginine- and cystine-rich and lacked free sulfhydryl groups and detectable levels of carbohydrate. Complete sequence determinations revealed that MCP-1 differed from MCP-2 only by the substitution of arginine for leucine at residue 13 from the NH2 terminus and that the molecules were each single chain polypeptides of 33 amino acid residues containing three intramolecular disulfide bonds. The complete amino acid sequences of MCP-1 and MCP-2 are: (sequence in text)
兔肺泡巨噬细胞的杀菌肽MCP - 1和MCP - 2通过一种改进的方法进行纯化，该方法采用制备性凝胶电泳和高效液相色谱。这些肽富含精氨酸和胱氨酸，缺乏游离巯基和可检测水平的碳水化合物。完整的序列测定表明，MCP - 1与MCP - 2的区别仅在于从NH2末端起第13位残基处亮氨酸被精氨酸取代，并且这些分子均为含33个氨基酸残基的单链多肽，含有三个分子内二硫键。MCP - 1和MCP - 2的完整氨基酸序列如下：（文中序列）
