兔白细胞来源的天然肽抗生素MCP-1和MCP-2对病毒的直接灭活作用。
Direct inactivation of viruses by MCP-1 and MCP-2, natural peptide antibiotics from rabbit leukocytes.
作者信息
Lehrer R I, Daher K, Ganz T, Selsted M E
出版信息
J Virol. 1985 May;54(2):467-72. doi: 10.1128/JVI.54.2.467-472.1985.
Abstract
Six homologous peptides were purified to homogeneity from rabbit granulocytes or alveolar macrophages and tested for their ability to inactivate herpes simplex virus type 1 (HSV-1). Two of the peptides, MCP-1 and MCP-2, showed considerable in vitro neutralizing activity, whereas four structurally homologous peptides (NP-3a, NP-3b, NP-4, and NP-5) were relatively ineffective. Inactivation of HSV-1 by MCP-1 or MCP-2 depended on peptide concentration and on the time, temperature, and pH of the incubation. HSV-2, vesicular stomatitis virus, and influenza virus A/WSN were also susceptible to direct neutralization by MCP-1 or MCP-2, whereas cytomegalovirus, echovirus type 11, and reovirus type 3 were not. We speculate that MCP-1 and MCP-2, peptides that are abundant in rabbit granulocytes and lung macrophages, may contribute to antiviral defenses by mediating the direct inactivation of HSV-1 and selected other viruses.
摘要
从兔粒细胞或肺泡巨噬细胞中纯化出六种同源肽并使其达到均一性，然后检测它们灭活1型单纯疱疹病毒（HSV-1）的能力。其中两种肽，即MCP-1和MCP-2，表现出相当强的体外中和活性，而四种结构同源肽（NP-3a、NP-3b、NP-4和NP-5）则相对无效。MCP-1或MCP-2对HSV-1的灭活取决于肽的浓度以及孵育的时间、温度和pH值。HSV-2、水疱性口炎病毒和甲型流感病毒A/WSN也易被MCP-1或MCP-2直接中和，而巨细胞病毒、11型艾柯病毒和3型呼肠孤病毒则不然。我们推测，在兔粒细胞和肺巨噬细胞中大量存在的MCP-1和MCP-2肽，可能通过介导HSV-1及其他某些病毒的直接灭活而有助于抗病毒防御。
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本文引用的文献
1
THE PATHOGENESIS OF HERPES VIRUS ENCEPHALITIS. II. A CELLULAR BASIS FOR THE DEVELOPMENT OF RESISTANCE WITH AGE.疱疹病毒性脑炎的发病机制。II. 随年龄增长产生抵抗力的细胞基础。
J Exp Med. 1964 Sep 1;120(3):359-74. doi: 10.1084/jem.120.3.359.
2
AMINO ACID REQUIREMENTS OF HERPES SIMPLEX VIRUS IN HUMAN CELLS.人细胞中单纯疱疹病毒的氨基酸需求
J Bacteriol. 1964 Mar;87(3):609-13. doi: 10.1128/jb.87.3.609-613.1964.
3
Microbicidal cationic proteins in rabbit alveolar macrophages: a potential host defense mechanism.兔肺泡巨噬细胞中的杀菌阳离子蛋白：一种潜在的宿主防御机制。
Infect Immun. 1980 Oct;30(1):180-92. doi: 10.1128/iai.30.1.180-192.1980.
4
Increased content of microbicidal cationic peptides in rabbit alveolar macrophages elicited by complete Freund adjuvant.完全弗氏佐剂诱导兔肺泡巨噬细胞中杀菌阳离子肽含量增加。
Infect Immun. 1981 Sep;33(3):775-8. doi: 10.1128/iai.33.3.775-778.1981.
5
Purification and antibacterial activity of antimicrobial peptides of rabbit granulocytes.兔粒细胞抗菌肽的纯化及其抗菌活性
Infect Immun. 1984 Jul;45(1):150-4. doi: 10.1128/iai.45.1.150-154.1984.
6
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7
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8
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Infect Immun. 1982 Jul;37(1):40-5. doi: 10.1128/iai.37.1.40-45.1982.
9
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