高效液相色谱法测定血清中的胺碘酮及其N-去乙基代谢物

Determination of amiodarone and its N-deethylated metabolite in serum by high-performance liquid chromatography.

作者信息

Mostow N D, Noon D L, Myers C M, Rakita L, Blumer J L

出版信息

J Chromatogr. 1983 Oct 14;277:229-37. doi: 10.1016/s0378-4347(00)84840-9.

Abstract

A high-performance liquid chromatographic (HPLC) method utilizing hexane extraction and a normal bonded phase column (NH2-alkylamine) was developed to measure serum concentrations of amiodarone and its N-deethylated metabolite. A single analysis requires 8 min. The one-step extraction efficiencies of amiodarone and the internal standard are greater than 90%. The method is linear between 0.05 and 20.0 micrograms/ml. The average relative standard deviation of the slope of the standard curve is 4% and the single day coefficient of variation is 3.2%. The use of hexane extraction for sample cleanup and a bonded phase column for chromatography result in a sensitive and reproducible system well suited to laboratories monitoring serum concentrations of multiple drugs by HPLC. A preliminary study has shown the assay to be useful for the investigation of the pharmacokinetics of this agent.

摘要

开发了一种利用己烷萃取和正相键合相柱（NH2 - 烷基胺）的高效液相色谱（HPLC）方法，用于测定血清中胺碘酮及其N - 去乙基代谢物的浓度。单次分析需要8分钟。胺碘酮和内标的一步萃取效率大于90%。该方法在0.05至20.0微克/毫升之间呈线性。标准曲线斜率的平均相对标准偏差为4%，单日变异系数为3.2%。使用己烷萃取进行样品净化以及使用键合相柱进行色谱分析，形成了一个灵敏且可重复的系统，非常适合通过HPLC监测多种药物血清浓度的实验室。一项初步研究表明该测定法可用于该药物的药代动力学研究。