Aitken J M, Thornley P E
J Clin Microbiol. 1983 Nov;18(5):1262-3. doi: 10.1128/jcm.18.5.1262-1263.1983.
Branhamella catarrhalis was suggested by the presence of gram-negative intracellular diplococci and confirmed by quantitative culture of the expectorated sputa of 11 patients with clinical evidence of lower respiratory tract infection. In quantitative culture, more than 20 colonies of B. catarrhalis were seen in each of the liquefied expectorated sputa at a dilution of 10(-7). Transtracheal aspirations were then performed on these 11 patients, and B. catarrhalis was isolated from the lower respiratory tract secretions of 10 of the patients. Of the 10 isolates, 5 produced beta-lactamase. None of the isolates grew on modified Thayer-Martin medium. The presence of gram-negative intracellular diplococci and the growth of B. catarrhalis on quantitative culture of expectorated sputum reliably indicates the presence of B. catarrhalis in the lower respiratory tract.
根据革兰氏阴性细胞内双球菌的存在怀疑为卡他布兰汉菌，并通过对11例有下呼吸道感染临床证据患者的咳出痰液进行定量培养得以证实。在定量培养中，在稀释度为10(-7)的每份液化咳出痰液中均可见到20个以上的卡他布兰汉菌菌落。然后对这11例患者进行经气管抽吸，从10例患者的下呼吸道分泌物中分离出卡他布兰汉菌。在这10株分离菌中，5株产生β-内酰胺酶。所有分离菌在改良的Thayer-Martin培养基上均不生长。咳出痰液定量培养中革兰氏阴性细胞内双球菌的存在以及卡他布兰汉菌的生长可靠地表明下呼吸道存在卡他布兰汉菌。
