Trotta P, Fox F, Krim M
J Biol Response Mod. 1983;2(4):348-59.
We report here on the development of (a) a double-antibody radioimmunoassay and (b) a solid-phase radioimmunoassay for cloned human interferon-alpha (leukocyte) [HuIFN-alpha(Le)]. We present the results of titrations of human interferon-alpha-2 (HuIFN-alpha 2) using either method under experimental and optimized conditions. A comparative study of the two methods indicates that (a) the double-antibody procedure is 200-fold more economical of antibody when quantitations are carried out within an optimal range of 0.05--1.0 ng; (b) the double-antibody method is fivefold more sensitive than the solid-phase method, its sensitivity being within the range of the antiviral assays for interferons; and (c) the solid-phase assay is significantly faster. The data also support the presence in human serum of a factor(s) that decreases the maximal amount of HuIFN-alpha 2 bound to antibody. We conclude that these radioimmunoassays are superior to biological assays for the quantitation of interferon-alpha polypeptides from the standpoints of objectivity and reproducibility as well as time and effort required.
我们在此报告关于（a）一种双抗体放射免疫测定法和（b）一种用于克隆人α干扰素（白细胞）[HuIFN-α(Le)]的固相放射免疫测定法的研发情况。我们展示了在实验和优化条件下使用这两种方法对人α干扰素-2（HuIFN-α 2）进行滴定的结果。对这两种方法的比较研究表明：（a）当在0.05--1.0 ng的最佳范围内进行定量时，双抗体程序在抗体使用上要节省200倍；（b）双抗体法比固相法灵敏五倍，其灵敏度在干扰素抗病毒测定范围内；（c）固相测定法明显更快。数据还支持人血清中存在一种能减少与抗体结合的HuIFN-α 2最大量的因子。我们得出结论，从客观性、可重复性以及所需时间和精力的角度来看，这些放射免疫测定法在定量α干扰素多肽方面优于生物学测定法。
