一种在扫描电子显微镜（SEM）制备过程中避免细胞收缩的简单方法的应用。
The use of a simple method to avoid cell shrinkage during SEM preparation.
作者信息
Wollweber L, Stracke R, Gothe U
出版信息
J Microsc. 1981 Feb;121(Pt 2):185-9. doi: 10.1111/j.1365-2818.1981.tb01211.x.
Abstract
In the course of preparing specimens for scanning electron microscopy, both glutaraldehyde and OsO4-fixed cells exhibit a considerable shrinkage with a reduction of the mean cellular diameter of about 45% after critical point drying. However, if cells are successively treated with glutaraldehyde, OsO4, tannic acid and uranyl acetate solutions, cellular shrinkage of only 5% is observed.
摘要
在为扫描电子显微镜制备标本的过程中，戊二醛和四氧化锇固定的细胞在临界点干燥后均表现出相当程度的收缩，平均细胞直径减小约45%。然而，如果细胞先后用戊二醛、四氧化锇、单宁酸和醋酸铀酰溶液处理，则仅观察到5%的细胞收缩。
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