Reyhani F, Nazarian I, Shakibi J
Jpn Heart J. 1983 Jul;24(4):607-14. doi: 10.1536/ihj.24.607.
To investigate the teratogenic effects of influenza virus on cardiac development two strains of influenza virus (Ty/Calif/5142/66 and Ty/Calif/64/M. Meleagrium) were injected into the yolk sac of fertile white Leghorn eggs during stage 23. Eggs injected with normal saline served as controls. The number of live embryos was significantly reduced in eggs injected with the 5142 strain, whereas the Meleagrium strain did not affect the number of live embryos in a significant manner. Serial sections of the live embryo hearts, extracted during stage 35, showed no cardiac anomalies in control groups. However, cardiac malformations were increased significantly in the embryos infected with the influenza viruses. A comparison of the two viral strains showed that the 5142 strain produced significantly more abnormal hearts than the Meleagrium strain. We conclude that (1) influenza infection during critical stages of embryonic development can lead to the cardiac anomalies, and (2) the virulence of a viral strain has a significant bearing on its teratogenic potential, at least in chick embryo hearts.
为研究流感病毒对心脏发育的致畸作用，在第23阶段将两种流感病毒株（Ty/Calif/5142/66和Ty/Calif/64/雉鸡）注射到可育白来航鸡卵的卵黄囊中。注射生理盐水的卵作为对照。注射5142株病毒的卵中活胚胎数量显著减少，而雉鸡株病毒对活胚胎数量无显著影响。在第35阶段提取的活胚胎心脏连续切片显示，对照组未发现心脏异常。然而，感染流感病毒的胚胎中心脏畸形显著增加。两种病毒株的比较表明，5142株病毒导致的心脏异常明显多于雉鸡株病毒。我们得出结论：（1）胚胎发育关键阶段的流感感染可导致心脏异常，（2）至少在鸡胚心脏中，病毒株的毒力对其致畸潜力有显著影响。
