溶葡萄球菌酶在耐溶葡萄球菌酶金黄色葡萄球菌突变体细菌细胞上的高效吸附

Efficient adsorption of lysostaphin on bacterial cells of lysostaphin-resistant Staphylococcus aureus mutant.

作者信息

Sakurada J, Murai M, Zhijun L, Usui A, Seki K, Kobayashi K, Sumi Y, Jitsukawa H, Masuda S

机构信息

Department of Bacteriology, Jikei University School of Medicine, Tokyo, Japan.

出版信息

Microbiol Immunol. 1993;37(1):29-34. doi: 10.1111/j.1348-0421.1993.tb03175.x.

DOI:10.1111/j.1348-0421.1993.tb03175.x

PMID:8474355

Abstract

A simple and efficient method for the purification of staphylolytic endopeptidase (lysostaphin) contained in culture supernatant of Staphylococcus simulans biovar staphylolyticus strain by adsorption of the enzyme on bacterial cells of lysostaphin-resistant S. aureus mutant was successfully devised. Lysostaphin was sufficiently absorbed on the heat-killed mutant cells derived from S. aureus Cowan I and efficiently eluted by 3 M KSCN. Enzyme preparation obtained by a single procedure of the affinity purification was pure enough for practical use. The yield of the enzyme was 25 mg from 1 liter culture and recovery rate was 64%.

摘要

成功设计出一种简单有效的方法，通过将溶葡萄球菌内肽酶（溶葡萄球菌素）吸附在耐溶葡萄球菌素的金黄色葡萄球菌突变体的细菌细胞上，来纯化模拟葡萄球菌生物变种溶葡萄球菌菌株培养上清液中所含的溶葡萄球菌内肽酶。溶葡萄球菌素能充分吸附在源自金黄色葡萄球菌考恩I型的热灭活突变体细胞上，并能用3M硫氰酸钾有效洗脱。通过单次亲和纯化程序获得的酶制剂纯度足以用于实际应用。每升培养物中酶的产量为25毫克，回收率为64%。