Ivanova G S, Petrikevich S B
Mikrobiologiia. 1983 Jul-Aug;52(4):573-8.
The localization of ribonucleases in Aspergillus clavatus mycelium was studied using differential centrifugation of mycelial homogenates, preparation of protoplasts, by cytochemical and immunochemical studies. The RNase activity was represented mainly by guanylic and nonspecific RNases and was not associated with the intracellular structures. The cytochemical and immunochemical observations revealed the presence of a nonspecific RNase activity in the periplasm, mitochondria, perinuclear space and vesicular structures. The presence of this RNase in the periplasmic space and vesicular structures of A. clavatus suggests that it may be secreted through a fusion of the vesicular membranes with the cytoplasmic membrane, and then from the periplasm through the cell wall into the surrounding medium.
利用菌丝体匀浆的差速离心、原生质体制备，通过细胞化学和免疫化学研究，对棒曲霉菌丝体中核糖核酸酶的定位进行了研究。核糖核酸酶活性主要由鸟苷酸酶和非特异性核糖核酸酶代表，且与细胞内结构无关。细胞化学和免疫化学观察显示，在周质、线粒体、核周间隙和囊泡结构中存在非特异性核糖核酸酶活性。棒曲霉周质空间和囊泡结构中存在这种核糖核酸酶，表明它可能通过囊泡膜与细胞质膜融合后分泌，然后从周质通过细胞壁进入周围介质。
