Beletskaia O P, Ivanova G S
Prikl Biokhim Mikrobiol. 1980 Jul-Aug;16(4):554-60.
A scheme for isolating guanyl-specific and -nonspecific intracellular and extracellular RNases from the fungus Aspergillus clavatus cultivated in a fermenter has been developed. The scheme involves chromatography on DEAE and CM-celluloses, QAE Sephadex A-50 gel filtration on Sephadex G-75 and ultragel A-44, and crystallization. Using the scheme, four electrophoretically homogeneous enzymes have been purified, two of them being crystalline guanyl-specific RNases and two nonspecific RNases.
已开发出一种从发酵罐中培养的棒曲霉中分离鸟苷特异性和非特异性细胞内及细胞外核糖核酸酶的方案。该方案包括在DEAE和CM-纤维素上进行色谱分离、在Sephadex G-75和Ultragel A-44上进行QAE Sephadex A-50凝胶过滤以及结晶。使用该方案已纯化出四种电泳纯的酶，其中两种是结晶鸟苷特异性核糖核酸酶，两种是非特异性核糖核酸酶。
