Fjose A, Pryme I F, Lillehaug J R
Mol Cell Biochem. 1983;56(2):137-44. doi: 10.1007/BF00227214.
After transfer of Krebs II ascites cells from the mouse peritoneum to suspension culture addition of the phorbol ester 12-0-tetradecanoyl-phorbol-13-acetate (TPA) causes an early stimulation of 3H-choline incorporation into phosphatidylcholine (PC). Choline transport into the treated cells, however, was unaffected. Within 30 min of TPA treatment 3H-choline incorporation was almost 300% above the control level. During a 5 hr period of suspension culture the overall patterns of 3H-choline incorporation were similar in TPA-treated and control cultures though the rate was greatly accentuated by the presence of the phorbol ester. Incubation of cells with cycloheximide prior to incubation with TPA did not result in an inhibition of the TPA-directed 3H-choline incorporation. After 3 hr incubation with TPA there were large increases in radioactivity in all subcellular fractions. At 20 hr, however, the values were not far from those of the control. During the first 3 hr of incubation with TPA the incorporation of 3H-choline into light rough (LR) and smooth (S) membranes was stimulated to levels of 400% and 320% respectively above control values. At later times the profiles of radioactivity in membrane subfractions in TPA-treated and control cultures were similar. The results illustrate an early effect of TPA on PC biosynthesis in Krebs II ascites cells while at later times of incubation the stimulatory effect was virtually abolished.
将克雷布斯II腹水癌细胞从小鼠腹膜转移至悬浮培养后，添加佛波酯12 - O -十四烷酰佛波醇-13 -乙酸酯（TPA）会导致3H -胆碱掺入磷脂酰胆碱（PC）的过程在早期受到刺激。然而，胆碱向经处理细胞内的转运未受影响。在TPA处理30分钟内，3H -胆碱掺入量比对照水平高出近300%。在5小时的悬浮培养期间，TPA处理组和对照组培养物中3H -胆碱掺入的总体模式相似，不过佛波酯的存在极大地加快了掺入速率。在用TPA孵育细胞之前先用环己酰亚胺孵育细胞，并未导致对TPA引导的3H -胆碱掺入的抑制。用TPA孵育3小时后，所有亚细胞组分中的放射性都大幅增加。然而，在20小时时，这些值与对照值相差不大。在与TPA孵育的最初3小时内，3H -胆碱掺入轻度粗面（LR）和滑面（S）膜的量分别被刺激至比对照值高400%和320%的水平。在随后的时间里，TPA处理组和对照组培养物中膜亚组分的放射性分布相似。结果表明TPA对克雷布斯II腹水细胞中PC生物合成有早期作用，而在孵育后期这种刺激作用几乎消失。
