Pryme I F, Lillehaug J R, Fjose A, Kleppe K
FEBS Lett. 1983 Feb 7;152(1):17-20. doi: 10.1016/0014-5793(83)80472-4.
12-O-Tetradecanoyl-phorbol-13-acetate (TPA) had a dual effect on the cellular membranes of C3H/10T1/2 cells in that it caused both a stimulation of [3H]choline incorporation and an enhancement of the solubilization of choline from prelabelled cells. Subfractionation studies showed that the release of [3H]choline occurred almost exclusively from nuclear-associated endoplasmic reticulum. The release was dependent on the presence of Mg2+ and Ca2+, indicating an enzyme-mediated reaction. In vivo, TPA stimulated the incorporation of [3H]choline into all subcellular fractions. The data indicate that the nuclear-associated endoplasmic reticulum represents an early target for TPA action.
12 - 十四酰佛波醇 - 13 - 乙酸酯（TPA）对C3H/10T1/2细胞的细胞膜有双重作用，它既刺激了[3H]胆碱的掺入，又增强了预先标记细胞中胆碱的溶解。亚分级研究表明，[3H]胆碱的释放几乎完全来自与核相关的内质网。这种释放依赖于Mg2+和Ca2+的存在，表明这是一个酶介导的反应。在体内，TPA刺激[3H]胆碱掺入所有亚细胞组分。数据表明，与核相关的内质网是TPA作用的早期靶点。
