Gitel'man A K, Ivanova V T, Zaktel'skaia L Ia, Bukrinskaia A G
Vopr Virusol. 1978 Mar-Apr(2):142-5.
The sedimentation and density properties of ribonucleoproteins were studied with different strains of influenza type A virus: A/turkey/Wisconsin/66, A/swine/Iowa/15/31, A/horse/Prague/56, antigenic (A/H3Neq1) and biological (MRC-11) recombinants of the A/Port-Chalmers/1/73 strain. In all the viruses under study, ribonucleoprotein (RNP) was found to be heterogeneous and to sediment in a zone of 30-70 S. Variability in the distribution of fragments in different strains was revealed. In A/turkey/Wisconsin/66 and MPC-11 strains the highest radioactivity was found in the zone of "heavy" fragments (60-70 S), in A/horse/Prague/56 and A/swine/Iowa/15/31 strains it was prevalent in the zone of 40-50 S. In recombinant A(H3Neq1) the distribution of radioactivity varied in different tests. The buoyant density in cesium chloride in all RNP compenents was found to be similar, 1.34-1.36 g/ml.
A/火鸡/威斯康星/66、A/猪/爱荷华/15/31、A/马/布拉格/56、A/波特-查尔默斯/1/73株的抗原性（A/H3Neq1）和生物学（MRC-11）重组体。在所有研究的病毒中，发现核糖核蛋白（RNP）是异质的，沉降在30-70S的区域。揭示了不同毒株中片段分布的变异性。在A/火鸡/威斯康星/66和MPC-11毒株中，“重”片段（60-70S）区域的放射性最高，在A/马/布拉格/56和A/猪/爱荷华/15/31毒株中，放射性在40-50S区域最为普遍。在重组体A（H3Neq1）中，不同试验中放射性的分布有所不同。发现所有RNP组分在氯化铯中的浮力密度相似，为1.34-1.36g/ml。
