用电锯切割冷冻的人类死后大脑导致儿茶酚胺含量降低。

Decreased catecholamine content caused by slicing of frozen human post mortem brain with an electric blade.

作者信息

Langlais P J, Walsh F X, Stevens T J, Bird E D

出版信息

Neurosci Lett. 1983 Oct 31;41(1-2):99-103. doi: 10.1016/0304-3940(83)90229-x.

Abstract

A deleterious effect on dopamine and norepinephrine levels was observed in cortical Brodmann area 25 tissue dissected from mechanically cut coronal slices of frozen human brain. Catecholamine concentrations were found to be 10 (NE) and 7 (DA) fold higher in similar tissue dissected directly from the frozen hemisphere without cutting. A heat-related breakdown in endogenous catecholamine content is suspected. An alternative method is presented for cutting coronal slices of frozen human brain which involves much less heat and friction and provides slices with excellent morphological appearance.

摘要

在从冷冻人脑机械切割冠状切片中分离出的皮质布罗德曼25区组织中，观察到多巴胺和去甲肾上腺素水平受到有害影响。发现直接从冷冻半球分离出的类似组织中，儿茶酚胺浓度分别比切割后的组织高10倍（去甲肾上腺素）和7倍（多巴胺）。怀疑存在与热相关的内源性儿茶酚胺含量分解。本文介绍了一种切割冷冻人脑冠状切片的替代方法，该方法产生的热量和摩擦力要小得多，并能提供形态良好的切片。