Ceder O, Roomans G M
Ultrastruct Pathol. 1983 Jun;4(4):305-9. doi: 10.3109/01913128309140583.
The effect of culture medium from fibroblast cultures of cystic fibrosis (CF) patients and healthy controls on the elemental composition of fibroblasts was investigated by X-ray microanalysis. Exposure of normal fibroblasts to culture medium from CF fibroblasts caused an increase in calcium level. Exposure of CF fibroblasts to culture medium from normal cells caused an increase of the sodium content of CF cells to approximately normal levels; the calcium level of the CF fibroblasts, however, remained abnormally high. The results may indicate that CF fibroblasts lack a factor needed for the regulation of sodium transport. CF fibroblast medium apparently contains a factor that interferes with the regulation of calcium transport.
通过X射线微量分析研究了囊性纤维化（CF）患者和健康对照者成纤维细胞培养的培养基对成纤维细胞元素组成的影响。正常成纤维细胞暴露于CF成纤维细胞的培养基中会导致钙水平升高。CF成纤维细胞暴露于正常细胞的培养基中会使CF细胞的钠含量增加到大约正常水平；然而，CF成纤维细胞的钙水平仍然异常高。结果可能表明CF成纤维细胞缺乏调节钠转运所需的一种因子。CF成纤维细胞培养基显然含有一种干扰钙转运调节的因子。
