von Euler A M, Ceder O, Kollberg H, Roomans G M
Exp Mol Pathol. 1985 Aug;43(1):142-9. doi: 10.1016/0014-4800(85)90064-4.
The effect of chronic treatment with cystic fibrosis (CF) fibroblast medium on rat submandibular gland and pancreas was investigated. Rats were injected for 8 days with conditioned medium from normal or CF fibroblasts. The elemental content of the acinar cells was measured by X-ray microanalysis of cryosections. A significant increase in cellular calcium, and a decrease in cellular sodium concentrations were found after treatment with CF medium. The ultrastructure of the submandibular acinar cells was not affected by the conditioned CF fibroblast culture medium. No effect of treatment with CF medium on ultrastructure and elemental content of pancreatic acinar cells could be demonstrated. The response to alpha-adrenergic, beta-adrenergic, cholinergic, and peptidergic stimulation in submandibular gland acinar cells of rats injected with normal or CF medium was investigated in vitro. With regard to changes in elemental composition after stimulation, no significant differences in response between the two groups could be found. Apparently, a factor in conditioned medium from cultured CF fibroblasts induces a net increase in calcium content of rat submandibular gland acinar cells. Possibly, this factor acts in a similar way in CF patients and may cause elevated calcium levels in CF cells.
研究了用囊性纤维化（CF）成纤维细胞培养基长期处理对大鼠下颌下腺和胰腺的影响。给大鼠注射来自正常或CF成纤维细胞的条件培养基，持续8天。通过对冰冻切片进行X射线微分析来测量腺泡细胞的元素含量。用CF培养基处理后，发现细胞内钙显著增加，细胞内钠浓度降低。下颌下腺腺泡细胞的超微结构不受CF成纤维细胞条件培养基的影响。未证明CF培养基处理对胰腺腺泡细胞的超微结构和元素含量有影响。在体外研究了注射正常或CF培养基的大鼠下颌下腺腺泡细胞对α-肾上腺素能、β-肾上腺素能、胆碱能和肽能刺激的反应。关于刺激后元素组成的变化，两组之间未发现显著的反应差异。显然，培养的CF成纤维细胞条件培养基中的一种因子会导致大鼠下颌下腺腺泡细胞的钙含量净增加。这种因子可能在CF患者中以类似方式起作用，并可能导致CF细胞中的钙水平升高。
