McLeod B C, Viernes A, Sassetti R J
Artif Organs. 1983 Nov;7(4):443-9. doi: 10.1111/j.1525-1594.1983.tb04224.x.
Because it has been found that some plasma separator membranes can activate complement in serum, this study focuses on the behavior of complement during actual membrane plasmapheresis. Complement metabolism in subjects undergoing membrane plasma separation with three different prototypic devices was studied. Complement activation was judged by crossed immunoelectrophoretic analysis of C3 in plasma. A cellulose acetate separator employed with heparin as the anticoagulant caused extensive C3 conversion (up to 50%) in separated plasma. A polysulfone separator used with citrate, and a polyvinylchloride derivative separator employed with heparin plus citrate, caused only small amounts of C3 conversion (0-10%) in separated plasma. C3 conversion in the cellulose acetate separator was reduced when citrate was included in the anticoagulant regimen, whereas C3 conversion in the polyvinylchloride derivative separator was increased when citrate was omitted. Activation of complement by the membrane material may occur during membrane plasma separation. Selection of membranes judged weakly activating by in vitro screening and the use of citrate anticoagulation would appear to minimize complement activation in membrane plasma separators.
由于已发现一些血浆分离膜可激活血清中的补体，本研究聚焦于实际膜式血浆置换过程中补体的行为。研究了使用三种不同原型设备进行膜式血浆分离的受试者的补体代谢。通过对血浆中C3进行交叉免疫电泳分析来判断补体激活情况。以肝素作为抗凝剂使用的醋酸纤维素分离器导致分离出的血浆中C3大量转化（高达50%）。与柠檬酸盐一起使用的聚砜分离器以及与肝素加柠檬酸盐一起使用的聚氯乙烯衍生物分离器，仅导致分离出的血浆中少量C3转化（0 - 10%）。当抗凝方案中加入柠檬酸盐时，醋酸纤维素分离器中的C3转化减少，而当省略柠檬酸盐时，聚氯乙烯衍生物分离器中的C3转化增加。膜材料可能在膜式血浆置换过程中激活补体。选择经体外筛选判断为弱激活的膜以及使用柠檬酸盐抗凝似乎可使膜式血浆分离器中的补体激活降至最低。
