Seely R J, Krueger R D, Fahrney D E
Biochem Biophys Res Commun. 1983 Nov 15;116(3):1125-8. doi: 10.1016/s0006-291x(83)80259-9.
Methanobacterium thermoautotrophicum was grown in phosphate-limited chemostat cultures at a dilution rate corresponding to a doubling time of 13.2 h. The cyclic-2,3-diphospho-D-glycerate content of these cells was 8 to 10-fold lower than that of cells grown in batch cultures having a doubling time of 11.5 h. This metabolite accounted for 5% of cell dry weight during batch growth on 2 mM phosphate. In the chemostat the steady-state concentration of phosphate was 4 microM, showing that this methanogen is adapted to highly efficient growth at low phosphate concentrations. Since growth rates were similar in both cultures, the growth rate clearly does not depend on intracellular levels of cyclic-2,3-diphosphoglycerate.
嗜热自养甲烷杆菌在磷酸盐限制的恒化器培养物中生长，稀释率对应于13.2小时的倍增时间。这些细胞的环-2,3-二磷酸-D-甘油酸含量比在倍增时间为11.5小时的分批培养中生长的细胞低8至10倍。在以2 mM磷酸盐进行分批生长期间，这种代谢物占细胞干重的5%。在恒化器中，磷酸盐的稳态浓度为4 microM，表明这种产甲烷菌适应于在低磷酸盐浓度下高效生长。由于两种培养物中的生长速率相似，生长速率显然不依赖于细胞内环-2,3-二磷酸甘油酸的水平。
