细菌视紫红质光循环的光声光谱学

Photoacoustic spectroscopy of bacteriorhodopsin photocycle.

作者信息

Renard M, Thirion P, Delmelle M

出版信息

Biophys J. 1983 Nov;44(2):211-8. doi: 10.1016/S0006-3495(83)84293-3.

DOI:10.1016/S0006-3495(83)84293-3

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1434823/

Abstract

Photoacoustic spectroscopy was applied to study the energetics and the kinetics of the slow intermediates of the bacteriorhodopsin photocycle. An analysis of the modulation frequency dependence of the photoacoustic signal allowed us to estimate the enthalpy changes and the kinetic parameters associated with those intermediates. The effects of pH, salt concentration, and protein aggregation were studied. Three photoacoustic transitions were found. The two low frequency transitions were attributed to O660 and M412, respectively. The third transition was interpreted as resulting from a protein conformational change undetected spectrophotometrically. The frequency spectra were simulated between 5 and 180 Hz at pH's 5.1, 7.0, and 8.9 assuming a branching in the bacteriorhodopsin photocycle at the M412 level. The enthalpy changes associated with M412 and O660 were computed and compared with the experimental values.

摘要

光声光谱法被用于研究细菌视紫红质光循环中慢中间体的能量学和动力学。对光声信号的调制频率依赖性进行分析，使我们能够估算与这些中间体相关的焓变和动力学参数。研究了pH值、盐浓度和蛋白质聚集的影响。发现了三个光声跃迁。两个低频跃迁分别归因于O660和M412。第三个跃迁被解释为是由分光光度法未检测到的蛋白质构象变化引起的。假设细菌视紫红质光循环在M412水平处有分支，在pH值为5.1、7.0和8.9时，对5至180Hz之间的频谱进行了模拟。计算了与M412和O660相关的焓变，并与实验值进行了比较。

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本文引用的文献

1

Photoacoustic calorimetry of purple membrane.紫膜的光声量热法。

Biophys J. 1982 Jan;37(1):4-6. doi: 10.1016/S0006-3495(82)84570-0.

2

Photoacoustic calorimetry of Halobacterium halobium photocycle.

Biochem Biophys Res Commun. 1980 Nov 17;97(1):200-6. doi: 10.1016/s0006-291x(80)80154-9.

3

Effect of Triton X-100 and of deuteration on the amplitude of the O640-intermediate in the bacteriorhodopsin photocycle.

FEBS Lett. 1981 Jun 15;128(2):249-54. doi: 10.1016/0014-5793(81)80092-0.

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The photochemical quantum yield of bacteriorhodopsin is pH independent: a photoacoustic study.

FEBS Lett. 1981 Jun 15;128(2):245-8. doi: 10.1016/0014-5793(81)80091-9.

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Quantum efficiency of light-driven proton extrusion in Halobacterium halobium. pH dependence.嗜盐菌中光驱动质子外排的量子效率。pH依赖性。

Biophys J. 1980 Dec;32(3):993-1006. doi: 10.1016/S0006-3495(80)85031-4.

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Biophys J. 1980 May;30(2):231-42. doi: 10.1016/S0006-3495(80)85091-0.

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Procedure for testing kinetic models of the photocycle of bacteriorhodopsin.细菌视紫红质光循环动力学模型的测试程序。

Biophys J. 1982 May;38(2):161-74. doi: 10.1016/S0006-3495(82)84543-8.

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Photoacoustic photocalorimetry and spectroscopy of Halobacterium halobium purple membranes.嗜盐菌紫膜的光声光量热法与光谱学

Biophys J. 1982 Feb;37(2):405-15. doi: 10.1016/S0006-3495(82)84686-9.

9

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Annu Rev Biophys Bioeng. 1981;10:315-54. doi: 10.1146/annurev.bb.10.060181.001531.

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Bacteriorhodopsin: a light-driven proton pump in Halobacterium Halobium.细菌视紫红质：嗜盐菌中的一种光驱动质子泵。

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