压力和温度对细菌视紫红质光循环中M412中间体的影响。对紫膜相变的启示。
Effects of pressure and temperature on the M412 intermediate of the bacteriorhodopsin photocycle. Implications for the phase transition of the purple membrane.
作者信息
Tsuda M, Govindjee R, Ebrey T G
出版信息
Biophys J. 1983 Nov;44(2):249-54. doi: 10.1016/S0006-3495(83)84296-9.
Abstract
The effects of pressure and temperature on the decay kinetics of the M412 (M) intermediate in the photocycle of bacteriorhodopsin were studied to provide information about the phase transitions of the purple membrane lipids. The activation volume (delta V++) for the decay of M is expected to be different below and above a phase transition. However, no abrupt change in delta V++ was found from 3.5 degrees to 60 degrees C. But a sharp break was observed in a plot of the logarithm of the rate of M decay vs. pressure. Extrapolation of this break point to standard atmospheric pressure gives a temperature of -42 degrees C, which probably corresponds to the phase transition of the purple membrane lipids. This conclusion is supported by studies of the effect of pressure on the M kinetics of bacteriorhodopsin incorporated into dimyristoylphosphatidylcholine vesicles, whose phase transition has previously been characterized.
摘要
研究了压力和温度对细菌视紫红质光循环中M412（M）中间体衰变动力学的影响，以提供有关紫膜脂质相变的信息。预计在相变温度以下和以上，M衰变的活化体积（ΔV++）会有所不同。然而，在3.5摄氏度至60摄氏度范围内未发现ΔV++有突然变化。但是，在M衰变速率的对数与压力的关系图中观察到一个急剧的转折点。将该转折点外推到标准大气压得到的温度为-42摄氏度，这可能对应于紫膜脂质的相变。将细菌视紫红质掺入二肉豆蔻酰磷脂酰胆碱囊泡中的M动力学的压力效应研究支持了这一结论，该囊泡的相变此前已得到表征。
相似文献
1
Effects of pressure and temperature on the M412 intermediate of the bacteriorhodopsin photocycle. Implications for the phase transition of the purple membrane.压力和温度对细菌视紫红质光循环中M412中间体的影响。对紫膜相变的启示。
Biophys J. 1983 Nov;44(2):249-54. doi: 10.1016/S0006-3495(83)84296-9.
2
Photoacoustic spectroscopy of bacteriorhodopsin photocycle.细菌视紫红质光循环的光声光谱学
Biophys J. 1983 Nov;44(2):211-8. doi: 10.1016/S0006-3495(83)84293-3.
3
Surface charge changes in purple membranes and the photoreaction cycle of bacteriorhodopsin.紫膜中的表面电荷变化与细菌视紫红质的光反应循环
Proc Natl Acad Sci U S A. 1980 Aug;77(8):4707-11. doi: 10.1073/pnas.77.8.4707.
4
Effect of partial delipidation of purple membrane on the photodynamics of bacteriorhodopsin.紫膜部分脱脂对细菌视紫红质光动力学的影响。
Biochemistry. 1990 Feb 27;29(8):1997-2002. doi: 10.1021/bi00460a006.
5
Bacteriorhodopsin in ice. Accelerated proton transfer from the purple membrane surface.冰中的细菌视紫红质。紫色膜表面质子转移加速。
FEBS Lett. 1990 Dec 17;277(1-2):277-80. doi: 10.1016/0014-5793(90)80864-f.
6
A low temperature investigation of the intermediates of the photocycle of light-adapted bacteriorhodopsin. Optical absorption and fluorescence measurements.光适应型细菌视紫红质光循环中间体的低温研究。光吸收和荧光测量。
Biochim Biophys Acta. 1979 Apr 11;546(1):106-20. doi: 10.1016/0005-2728(79)90174-9.
7
Transient photovoltages in purple membrane multilayers. Charge displacement in bacteriorhodopsin and its photointermediates.紫色膜多层结构中的瞬态光电压。细菌视紫红质及其光中间体中的电荷位移。
Biochim Biophys Acta. 1978 May 18;509(2):300-17. doi: 10.1016/0005-2736(78)90049-4.
8
Effect of the removal of the COOH-terminal region of bacteriorhodopsin on its light-induced H+ changes.细菌视紫红质COOH末端区域的去除对其光诱导的H⁺变化的影响。
Biophys J. 1982 Apr;38(1):85-7. doi: 10.1016/S0006-3495(82)84534-7.
9
[Luminescence of bacteriorhodopsin in purple membranes from Halobacterium haolbium cells].
Biofizika. 1976 Mar-Apr;21(2):313-20.
10
Photoacoustic photocalorimetry and spectroscopy of Halobacterium halobium purple membranes.嗜盐菌紫膜的光声光量热法与光谱学
Biophys J. 1982 Feb;37(2):405-15. doi: 10.1016/S0006-3495(82)84686-9.
引用本文的文献
1
Brighter than the sun: Rajni Govindjee at 80 and her fifty years in photobiology.比太阳更耀眼：80岁的拉吉尼·戈文吉及其在光生物学领域的五十年
Photosynth Res. 2015 Apr;124(1):1-5. doi: 10.1007/s11120-015-0106-0. Epub 2015 Mar 5.
2
Pressure effects on the dark-adaptation of bacteriorhodopsin.压力对菌紫质暗适应的影响。
Biophys J. 1993 Apr;64(4):1187-93. doi: 10.1016/S0006-3495(93)81484-X.
3
Temperature jump study of charge translocation during the bacteriorhodopsin photocycle.细菌视紫红质光循环过程中电荷迁移的温度跃变研究。
Biophys J. 1989 Nov;56(5):851-9. doi: 10.1016/S0006-3495(89)82731-6.
4
Branching photocycle of sensory rhodopsin in halobacterium halobium.嗜盐菌中感光视紫红质的分支光循环。
Biophys J. 1988 Apr;53(4):493-6. doi: 10.1016/S0006-3495(88)83128-X.
5
Pressure dependence of the photocycle kinetics of bacteriorhodopsin.细菌视紫红质光循环动力学的压力依赖性
Biophys J. 2002 Dec;83(6):3490-8. doi: 10.1016/S0006-3495(02)75348-4.
6
Kinetic and thermodynamic study of the bacteriorhodopsin photocycle over a wide pH range.在较宽pH范围内对细菌视紫红质光循环的动力学和热力学研究。
Biophys J. 1998 Dec;75(6):3110-9. doi: 10.1016/S0006-3495(98)77752-5.
7
Electron diffraction analysis of the M412 intermediate of bacteriorhodopsin.细菌视紫红质M412中间体的电子衍射分析。
Biophys J. 1986 Nov;50(5):913-20. doi: 10.1016/S0006-3495(86)83532-9.
本文引用的文献
1
Calorimetric and fluorescence depolarization studies on the lipid phase transition of bacteriorhodopsin--dimyristoylphosphatidylcholine vesicles.关于细菌视紫红质 - 二肉豆蔻酰磷脂酰胆碱囊泡脂质相变的量热法和荧光去极化研究。
Biochemistry. 1981 Dec 8;20(25):7109-15. doi: 10.1021/bi00528a009.
2
Effect of high pressure on the absorption spectrum and isomeric composition of bacteriorhodopsin.高压对细菌视紫红质吸收光谱和异构体组成的影响。
Biophys J. 1980 Apr;30(1):149-57. doi: 10.1016/S0006-3495(80)85083-1.
3
Lipid--protein interactions in bacteriorhodopsin--dimyristoylphosphatidylcholine vesicles.细菌视紫红质 - 二肉豆蔻酰磷脂酰胆碱囊泡中的脂 - 蛋白相互作用
Biochemistry. 1981 Feb 17;20(4):840-9. doi: 10.1021/bi00507a029.
4
High pressure effects on proteins and other biomolecules.高压对蛋白质及其他生物分子的影响。
Annu Rev Biophys Bioeng. 1982;11:1-21. doi: 10.1146/annurev.bb.11.060182.000245.
5
Proton and carbon-13 nuclear magnetic resonance studies of the polar lipids of Halobacterium halobium.嗜盐栖热菌极性脂质的质子和碳-13核磁共振研究。
Biochemistry. 1980 Apr 15;19(8):1626-31. doi: 10.1021/bi00549a016.
6
Pressure effect on the Arrhenius discontinuity in Ca2+-ATPase from sarcoplasmic reticulum: evidence for lipid involvement.
FEBS Lett. 1980 Aug 11;117(1):161-3. doi: 10.1016/0014-5793(80)80935-5.
7
The pressure dependence of the lipid bilayer phase transition.脂质双分子层相变的压力依赖性。
Biochemistry. 1974 Aug 13;13(17):3494-6. doi: 10.1021/bi00714a012.
8
Osmometric and microscopic studies on bilayers of polar lipids from the extreme halophile, Halobacterium cutirubrum.对极端嗜盐菌——红皮盐杆菌的极性脂质双层进行的渗透测定和显微镜研究。
Biochim Biophys Acta. 1974 Jun 13;352(2):202-17. doi: 10.1016/0005-2736(74)90212-0.
9
Improved isolation procedures for the purple membrane of Halobacterium halobium.嗜盐菌紫膜的改进分离程序。
Prep Biochem. 1975;5(2):161-78. doi: 10.1080/00327487508061568.
10
Kinetic isotope effects in the photochemical cycle of bacteriorhodopsin.细菌视紫红质光化学循环中的动力学同位素效应。
Biophys Struct Mech. 1976 Dec 22;2(3):267-76. doi: 10.1007/BF00535372.
Zotero 插件