Schmidt K, Bruchelt G, Kistler D, Koslowski L
Burns Incl Therm Inj. 1983 Nov;10(2):79-85. doi: 10.1016/0305-4179(83)90002-5.
Despite substantial progress in handling the acute phase, about 50 per cent of all severely burned patients are still subject to lethal infections during the later stages of the burn disease. Such patients frequently succumb to infections by opportunistic bacteria and viruses of normally low virulence indicating that the antiinfectious host defences are severely compromised. The present study was conducted in order to evaluate the effects of severe thermal injury on the two major categories of phagocytic cells, the circulating phagocytes of the blood and the alveolar macrophages as one population of the fixed phagocytes of the reticulo-endothelial system. The cells were isolated from burned and unburned rats. For quantitative assessment of the phagocytic function the chemiluminescence associated with the phagocytosis of opsonized zymosan particles was measured, using luminol as a chemiluminigenic probe. It turned out that as a consequence of the thermal trauma the phagocytic activity as measured by chemiluminescence is reduced in granulocytes as well as in alveolar macrophages.
尽管在处理急性期方面取得了重大进展，但所有严重烧伤患者中仍有大约50%在烧伤疾病后期会遭受致命感染。这类患者常常死于由通常低毒力的机会性细菌和病毒引起的感染，这表明抗感染的宿主防御功能严重受损。本研究旨在评估严重热损伤对两类主要吞噬细胞的影响，即血液中的循环吞噬细胞和作为网状内皮系统固定吞噬细胞群体之一的肺泡巨噬细胞。细胞取自烧伤和未烧伤的大鼠。为了定量评估吞噬功能，使用鲁米诺作为化学发光探针，测量与调理酵母聚糖颗粒吞噬作用相关的化学发光。结果表明，热创伤导致粒细胞和肺泡巨噬细胞中通过化学发光测量的吞噬活性降低。
