Kostić D
Adv Exp Med Biol. 1978;101:667-78. doi: 10.1007/978-1-4615-9071-2_62.
Disialoganglioside G2a was separated by one dimensional two-step TLC between the two ganglioside fractions using long plate precoated with silica H+ florisil in the particular solvent system. Behaviour of this fraction to the enzyme effect and molecule percentages of its components showed the characteristics of the ganglioside containing two N-acetylneuraminic acids. Synthesis dynamics of this fraction in rat brain was very intensive in the myelination period and it practically ended in 40th day. In mutant "Jimpy" mice brain ganglioside G2a served as an indicator of the normal ganglioside synthesis in relation to the changes in myelin sphingolipid molecular structure. Comparative brain studies undertaken in various species, intracellular rat brain fractions, various structures of Torpedo ocellata electric fish, emphasized the importance of this ganglioside in cell membrane formation where it is obligatory component. Quantitative determination of this fraction in Astrocytoma with various malignancy degree offers great help in differential diagnosis.
双唾液酸神经节苷脂G2a是在特定溶剂系统中，使用预先涂有硅胶H +弗罗里硅土的长板，通过一维两步薄层层析法在两种神经节苷脂组分之间分离得到的。该组分对酶作用的行为及其成分的分子百分比显示出含有两个N - 乙酰神经氨酸的神经节苷脂的特征。该组分在大鼠脑中的合成动力学在髓鞘形成期非常活跃，实际上在第40天结束。在突变的“Jimpy”小鼠脑中，神经节苷脂G2a作为正常神经节苷脂合成相对于髓鞘鞘脂分子结构变化的指标。在各种物种、大鼠脑细胞内组分、电鳐电鱼的各种结构中进行的脑比较研究强调了这种神经节苷脂在细胞膜形成中的重要性，它是细胞膜的必需成分。对不同恶性程度的星形细胞瘤中该组分的定量测定为鉴别诊断提供了很大帮助。
